摘要
目的对山东省境内首次发现的入侵生物福寿螺进行物种鉴定。方法2021年10月,在山东省济宁市野外采集福寿螺样本进行形态学鉴定;随机选取部分福寿螺样本,取螺腹足部肌肉组织提取基因组DNA进行多重PCR扩增并测序,利用MegAlign 7.1.0软件对测序后的序列进行比对并构建系统发育树。采用肺检法检测福寿螺中广州管圆线虫感染情况。结果共采集福寿螺活体样本104只,经形态学鉴定均为福寿螺属物种;随机挑选12只成螺样本,经多重PCR和测序鉴定11只为小管福寿螺、1只为斑点福寿螺。104只福寿螺均未发现广州管圆线虫感染。结论山东省境内首次发现福寿螺入侵,且存在小管福寿螺和斑点福寿螺。
Objective To characterize the species of invasive Pomacea snails that were discovered for the first time in Shandong Province.Methods Pomaceasnails samples were collected in the field of Jining City,Shandong Province on October2021 for morphological identification.Pomacea snails were randomly sampled and genomic DNA was extracted from foot muscle tissues of Pomacea snails for multiplex PCR amplification.The PCR amplification product was sequenced.Then,the sequence was aligned and a phylogenetic tree was created using the software MegAlign 7.1.0.In addition,Angiostongylus cantonensis infection was detected in Pomacea snails with the lung microscopy.Results A total of 104 living Pomacea snails were collected,and all were characterized as Pomacea spp.based on morphological features.Of 12 randomly selected adult Pomacea snails,multiplex PCR assay and sequencing identified eleven snails as P.canaliculata and one as P.maculata.No A.cantonensis infection was detected in 104 Pomacea snails.Conclusion where P.canaliculata and P.maculata are found.
作者
王龙江
许艳
孙慧
张本光
孔祥礼
韩海涛
李瑾
李曰进
杨丽敏
郭云海
王用斌
WANG Long-jiang;XU Yan;SUN Hui;ZHANG Ben-guang;KONG Xiang-li;HAN Hai-tao;LI Jin;LI Yue-jin;YANG Li-min;GUO Yun-hai;WANG Yong-bin(Shandong Institute of Parasitic Diseases,Shandong First Medical University&Shandong Academy of Medical Sciences,Jining,Shandong 272000,China;Yanzhou District Center for Disease Control and Prevention,Jining City,Shandong Province,China;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Min-istry of Science and Technology,National Health Commission Key Laboratory of Parasite and Vector Biology,Shanghai 200025,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2022年第4期407-411,共5页
Chinese Journal of Schistosomiasis Control
基金
国家重点研发计划项目(2021YFC2300800,2021YFC2300804)
山东省医药卫生科技发展计划项目(202112050099,202001050530,202001050586)
上海市公共卫生体系建设三年行动计划第五轮重点学科(GWV-10.1-XK13)。
关键词
福寿螺
广州管圆线虫
生物入侵
多重PCR
山东省
Pomaceasnail
Angiostongylus cantonensis
Biological invasion
Multiplex PCR assay
Shandong Province
