摘要
[目的]在对3.5年生青钱柳种质资源进行评价的基础上,初选适合南京及周边环境相似地区的优良家系及单株,为青钱柳定向培育提供理论依据。[方法]以青钱柳种质资源库31个家系为材料,测定其生长性状和叶总黄酮、总三萜、总多酚含量,分析各性状间的差异,并对青钱柳家系进行聚类分析,同时利用熵权法进行优良家系-单株配合选择。[结果]青钱柳树高、地径等生长性状及叶总黄酮、总三萜、总多酚含量在参试家系间存在显著差异;聚类分析将31个参试家系聚为4类,第1类推荐为高三萜积累家系,第2类推荐为高多酚积累家系,第3类推荐为高黄酮积累家系,第4类为叶次生代谢物综合含量表现较差家系。根据生长性状和叶主要次生代谢物含量的数据,采用熵权法初选出7个优良家系,分别为GXJZS7#、GZSQ12#、GXLS26#、HNYS2#、AHQLF13#、SCMC31#和GXJZS1#;针对优良家系内单株进一步进行综合评价,筛选出11株优良单株,在叶片总黄酮、总三萜和总多酚含量上综合表现较优。[结论]青钱柳生长和次生代谢物含量在家系间存在显著差异,为青钱柳良种选育提供了极大的空间;基于优良家系-单株配合选择,初步筛选出7个优良家系和11株优良单株可用于进一步试验和应用。
[Objective]In order to select superior families and individual plants which is suitable for planting in Nanjing and surrounding areas for providing a theoretical basis for the oriented silviculture of Cyclocarya paliurus plantations.[Method]The 3.5-year old germplasm resources of C.paliurus trees were comprehensively evaluated in this study.The 31 families of C.paliurus trees were used as test materials to determine the traits of growth and the leaf contents of total flavonoid,triterpenoid,and polyphenol.And cluster analysis and entropy method were used to screen superior families and individual trees based on the variations in traits.[Results]Significant variations were observed in tree height,diameter at the base,and the leaf contents of total flavonoid,triterpenoid and polyphenol among different families.Hierarchical cluster analysis showed that the 31 families of C.paliurus were classified into four distinct groups.The C.paliurus families in cluster 1,cluster 2 and cluster 3 could be recommended as the families for high accumulation of total triterpenoid,total polyphenol,and total flavonoid,respectively.In contrast,the families in cluster 4 showed poor performance in all measured metabolites.Seven superior families(GXJZS7#、GZSQ12#、GXLS26#、HNYS2#、AHQLF13#、SCMC31#and GXJZS1#)of C.paliurus were preliminary selected for the accumulation of target secondary metabolites using entropy method.Within the seven superior families,all the trees were further assessed,and eleven superior individuals were selected.[Conclusion]There are significant variations in growth and leaf secondary metabolites among the tested germplasm resources,showing a great potential for the selecting and breeding of C.paliurus.Based on the comprehensive assessment,seven superior families and eleven excellent individuals are selected for further test and future application.
作者
蓝丽霞
徐展宏
孙操稳
方升佐
LAN Li-xia;XU Zhan-hong;SUN Cao-wen;FANG Sheng-zuo(College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China;Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,Jiangsu,China)
出处
《林业科学研究》
CSCD
北大核心
2022年第5期42-51,共10页
Forest Research
基金
江苏省农业重点研发项目(No.BK20200771)
国家自然科学基金面上项目(32071750)。
关键词
青钱柳
生长
次生代谢物质
综合评价
Cyclocarya paliurus
growth
secondary metabolites
comprehensive assessment
