摘要
单核细胞增生李斯特氏菌普遍存在于自然界中,是一种常见食源性致病菌,属于人畜共患病,可引起严重的李斯特氏菌病。因此,对食品中单增李斯特氏菌的控制和监测极为重要,以便更好地预防食源性疾病的暴发。目前,检测单增李斯特氏菌的方法中应用较为广泛的是传统的分离鉴定、分子生物学检测和免疫学检测法。传统方法易于操作、灵敏度高、成本低,但检测周期长,没办法满足对目的食物快速检测的需求。免疫学检测方法时间短,步骤简单,但该方法要依赖较高特异性的抗体。分子学检测法快速准确,灵敏度高,但是该方法需要有丰富的操作经验,而且需要特殊仪器。论文综述了传统方法、免疫学检测技术、分子生物学检测技术等多种单增李斯特氏菌的检测方法,结合最新参考文献就检测研究进展予以分析,以期为食品中单增李斯特氏菌的快速检测研究提供参考。
Listeria monocytogenes is one of the common foodborne pathogenic bacteria widely existing in nature.At the same time,it will also lead to serious zoonosis.Therefore,it is very important to monitor and control Listeria monocytogenes in food in order to better prevent the outbreak of foodborne diseases.At present,the detection methods of Listeria monocytogenes are widely used in traditional isolation and identification,molecular detection and immunological detection.Traditional detection has strong operability,high sensitivity and low cost,but the detection cycle is long,which can not meet the needs of rapid detection of target food.The immunological detection time is short and the operation is simple,but the method depends on highly specific antibodies.Molecular detection method is time-saving,labor-saving and high sensitivity,but molecular detection method needs rich operation experience and is not suitable for on-site mass detection.This paper summarizes the detection methods of Listeria monocytogenes such as traditional detection,immunological detection and molecular biology detection methods,and analyzes the detection research progress combined with the latest literature,in order to provide reference for the rapid detection of Listeria monocytogenes in food.
作者
刘洪蕾
王真
LIU Hong-lei;WANG Zhen(Beijing University of Agriculture,Animal Science and Technology College,Beijing,102206,China)
出处
《动物医学进展》
北大核心
2022年第10期111-116,共6页
Progress In Veterinary Medicine
基金
北京市优秀人才培养资助项目(2017000026833ZK18)
2019年北京市属高校高水平教师队伍建设支持计划项目(CIT&TCD201904052)
北京农学院青年科学基金项目(QNKJ202107)
2021年北京农学院学位与研究生教育改革与发展项目(2021YJS030)。
关键词
单增李斯特氏菌
传统分离鉴定法
实时荧光定量PCR
免疫磁分离技术
Listeria monocytogenes
traditional isolation and identification method
quantitative real-time PCR
immunomagnetic beads
