摘要
为了解阿拉尔市部分即食食品中单增李斯特氏菌污染状况及对抗生素的敏感性,采取随机抽样法采集阿拉尔市超市、农贸市场及餐饮店等场所的熟肉制品、生食蔬菜、沙拉、甜点等即食食品360份,进行单增李斯特氏菌分离培养、鉴定及小鼠致病性试验,用圆纸片扩散法(K-B法)对鉴定菌株进行11种抗生素的药敏试验。结果显示,单增李斯特氏菌总检出率为6.39%,其中餐饮店的检出率最高为8.33%;按即食食品种类来说,熟肉制品污染情况最严重为8.89%;小鼠感染试验表明,分离株均有致病性;23株菌对11种抗生素总耐药率为21.7%,氯霉素和四环素的耐药率最高为8.7%,对头孢西丁、头孢噻吩、克林霉素、红霉素、青霉素和利福平均敏感。研究表明,阿拉尔市部分即食食品存在单增李斯特氏菌污染,且分离株均有致病性,对不同抗生素耐药性不同。
In order to understand the contamination of Listeria monocytogenes in some ready to eat foods and its sensitivity to antimicrobials in Alar city,360 cooked meat products,raw vegetables,salads,desserts and other ready to eat foods from supermarkets,farmers'markets and restaurants in Alar city were collected by random sampling.Listeria monocytogenes was isolated,cultured,identified and tested for pathogenicity in mice according to the qualitative detection method of GB/T 4789.30-2016,and the round paper diffusion method(K-B method)was used.The drug sensitivities of 11 antimicrobials were tested.The results showed that the total detection rate of Listeria monocytogenes was 6.39%,the highest detection rate in restaurants was 8.33%,and the pollution of cooked meat products was the most serious,8.89%;Mouse infection test showed that the isolates were pathogenic;The total resistance rate of 23 strains to 11 antimicrobials was 21.7%,the highest resistance rate of chloramphenicol and tetracycline was 8.7%,and they were sensitive to cefoxitin,cefotaxin,clindamycin,erythromycin,penicillin and rifampicin.The research showed that some ready to eat foods in Alar city are contaminated by Listeria monocytogenes,and the isolates have pathogenicity and different drug resistance.
作者
郑雅雯
韩慧
米热依·奴尔沙热普
巴合达吾列提·达吾列提汗
吴静
ZHENG Ya-wen;HAN Hui;Mireyi nursarep;Bahdavuleti davuleti Khan;WU Jing(College of Animal Science,Tarim University,Alar,Xinjiang,843300,China)
出处
《动物医学进展》
北大核心
2022年第9期49-52,共4页
Progress In Veterinary Medicine
基金
新疆生产建设兵团重点领域科技攻关计划项目(2020AB025)
塔里木大学大学生创新创业训练计划项目(202010757010)。
关键词
即食食品
单增李斯特氏菌
分离鉴定
致病性
药敏试验
ready to eat food
Listeria monocytogenes
isolation and identification
bacterial pathogenicity
drug sensitivity test
