摘要
目的:探讨艾片(左旋龙脑)、天然冰片(右旋龙脑)、冰片(合成龙脑)不同剂量对实验性急性心梗(AMI)模型大鼠心电生理及抗炎、调控缺氧诱导因子-1α(HIF-1α)/血管内皮生长因子(VEGF)心血管保护的可能机制。方法:SD雄性成年大鼠,按体重随机分为假手术组、模型组、溶剂模型组、硝酸甘油组、天然冰片高、中、低剂量(0.6、0.3、0.15 g·kg^(-1))组、艾片及合成冰片高、中、低剂量(0.2、0.1、0.05 g·kg^(-1))组,每组10只。按10 mL·kg^(-1)灌胃,预给药3 d,末次给药30 min后,施冠状动脉左前降支(LAD)结扎造模,将模型成功大鼠连续治疗3 d。采用BL-420N生物系统分析造模前后及治疗3 d后心电图及心率变异性(HRV),实时荧光定量聚合酶链式反应(Real-time PCR)检测心肌组织中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)mRNA表达量,蛋白免疫印迹法、免疫组织化学法检测血管内皮生长因子受体1(VEGFR1)、HIF-1α、CD34蛋白表达水平。结果:与假手术组比较,模型组造模当天及干预3 d后心率、心电ST段、T波、QRS时限、QTC间期、Q波显著升高,HRV及T波明显改变(P<0.05,P<0.01);与溶剂模型组比较,造模当天艾片中、低剂量组、天然冰片组心率,艾片组、天然冰片高、中剂量、冰片高剂量组ST波幅,艾片组、天然冰片中、低剂量、冰片高剂量组HRV参数,艾片高、中剂量、天然冰片低剂量、冰片组低频与高频范围内的功率谱比值(LF/HF),艾片、冰片高剂量、天然冰片中剂量组T波波幅,艾片中、低剂量、天然冰片高、中剂量组QTC间期,艾片与天然冰片各自高、低剂量、冰片组QRS时限,均明显减小或缩短(P<0.05,P<0.01);治疗3 d后艾片组、天然冰片高、中剂量、冰片中剂量组心率,艾片组、天然冰片高、中剂量、冰片高剂量组的ST段波幅,艾片与天然冰片各自高剂量、冰片高、中剂量组的QTC间期与QRS时限及Q波波幅,艾片中剂量组QRS
Objective:To investigate the possible mechanism of different doses of L-Borneolum,Borneolum,and Borneolum Syntheticum in the electrophysiology,anti-inflammation,and regulation of hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)cardiovascular protection of the experimental acute myocardial infarction(AMI)rats.Method:SD male adult rats were randomly divided into thirteen groups according to their body weight,namely the sham operation group,the model group,the solvent model group,the nitroglycerin group,the Borneolum high,medium,and low-dose(0.6,0.3,0.15 g·kg^(-1))groups,the L-Borneolum high,medium,and low-dose(0.2,0.1,0.05 g·kg^(-1))groups,and the Borneolum Syntheticum high,medium,and low-dose(0.2,0.1,0.05 g·kg^(-1))groups,with 10 rats in each group.Rats were given 10 mL·kg^(-1)by gavage for 3 d of pre-administration.Thirty minutes after the last administration,the left anterior descending coronary artery(LAD)was ligated to induce the model,and the successful rat model was continuously treated for 3 d.BL-420N biosystem was used to analyze the electrocardiogram(ECG)and heart rate variability(HRV)before and after modeling and after 3 d of treatment.Real-time quantitative polymerase chain reaction(Real-time PCR)was used to determine the mRNA expressions of interleukin-1β(IL-1β)and interleukin-6(IL-6)in the myocardial tissue Western blot and immunohistochemistry were used to determine the protein expression levels of VEGF receptor 1(VEGFR1),HIF-1α,and CD34.Result:Compared with the sham operation group,the model group significantly increased the heart rate,ECG ST wave,T wave,QRS duration,QTC interval,and Q wave on the day of modeling and after 3 d of treatment,and significantly changed HRV and T wave(P<0.05,P<0.01).As compared with the solvent model group,on the day of modeling,the heart rate of the L-Borneolum medium and low-dose groups and the Borneolum groups,the ST wave of the L-Borneolum groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high-dose gro
作者
王立映
王建
樊亚梅
王佳俊
付尹
杨显娟
龚道银
WANG Liying;WANG Jian;FAN Yamei;WANG Jiajun;FU Yin;YANG Xianjuan;GONG Daoyin(School of Pharmacy,Chengdu University of Traditional Chinese Medicine(TCM),Chengdu 611137,China;Hospital of Chengdu University of TCM,Chengdu 610075,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2022年第19期61-72,共12页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81873023)。
关键词
3种冰片
心肌梗死
心肌电生理
炎症因子
血管新生
3 kinds of Borneolum
myocardial infarction
myocardial electrophysiology
inflammatory factors
angiogenesis
