摘要
目的研究福建省2011—2020年手足口病(hand,foot and mouth disease,HFMD)相关的柯萨奇病毒A2型(coxackievirus A2,CV-A2)流行病学及病毒基因组特征。方法利用描述性流行病学方法对2011—2020年福建省疾病预防控制中心实验室收集的HFMD相关CV-A2病例进行流行病学特征分析。采用Mega X和Simplot 3.5.1等软件对一代测序获得的VP1基因和二代测序获得的全基因组进行系统进化分析和重组分析。结果2011—2020年福建省HFMD相关CV-A2病例35例,以1~2岁幼儿(28/35)为主,男女性别比2.5∶1(25/10)。VP1区基因系统进化树显示,福建省内CV-A2流行株存在两种基因亚型,即C1和D1基因亚型,仅1株(2019FJPT064)属于C1基因亚型,其余27株为D1基因亚型。其中,4株2011—2012年分离株为D1基因亚型cluster 1分支,另外2株2011—2012年分离株及2012年后的分离株均属于D1基因亚型cluster 2分支。6株福建CV-A2毒株全基因组长度在7393~7402bp之间,VP1区核苷酸序列与CV-A2原型株相似性为80.3%~81.7%,其他各片段与CV-A2原型株相似性72.7%~86.2%。P2区进化树显示6株分离株与柯萨奇病毒A4型(coxackievirus A4,CV-A4)进化距离较近。P3区进化树显示,2011FJFZ027与柯萨奇病毒A6型(coxackievirus A6,CV-A6)分离株、2012FJQZ311与柯萨奇病毒A14型(coxackievirus A14,CV-A14)分离株、2020FJPTN040与柯萨奇病毒A8型(coxackievirus A8,CV-A8)分离株进化距离近。重组分析显示,分离株2011FJFZ027、2012FJQZ311、2020FJPTN040在非结构蛋白区分别可能与CV-A6(GenBank登录号:KR706309)、CV-A14(GenBank登录号:KP036482)、CV-A8(GenBank登录号:KM609475、MT648786)流行株发生重组。结论2011—2020年福建省HFMD相关CV-A2流行强度呈现散发形式,D1基因亚型CV-A2在福建省内持续流行,从2011—2012年以D1基因亚型cluster 1分支为主转变为2012年以后的D1基因亚型cluster 2分支;福建省CV-A2流行株重组频繁,存在多种重组模式。
Objective To study the epidemiological and molecular characteristics of coxackievirus A2(CV-A2)associated with hand,foot and mouth disease(HFMD)in Fujian province,2011-2020.Methods Descriptive epidemiological method was used to analyze the epidemiological characteristics of CV-A2.Case information was collected in the laboratory of Fujian Provincial Center for Disease Control and Prevention from 2011 to 2020.The VP1 gene obtained by first-generation sequencing and the complete genome obtained by second-generation sequencing were made phylogenetic analysis and recombination analysis by Mega X and Simplot3.5.1.Results From 2011 to 2020,there were 35 HFMD-associated CV-A2 cases in Fujian province,most of whom aged 1 to 2 years(28/35),and the gender ratio of male to female was 2.5∶1(25/10).Phylogenetic tree of VP1 region showed that CV-A2 in Fujian province was mainly distributed in the evolutionary branches of D1 and C1 subgenotype,only one strain(2019FJPT064)belonged to C1 subgenotype and the other 27 strains were grouped under D1 subgenotype.Among them,4 isolates from 2011 to 2012 belonged to cluster 1 of D1 subgenotype,and the other two isolates from 2011 to 2012 and those after 2012 belonged to cluster 2 of D1 subgenotype.The complete genome length of 6 CV-A2 strains were about 7393 to 7402 bp.The homology of the nucleic acid sequences in the VP1 region of 6 CV-A2 strains and the CV-A2 prototype strain were 80.3%-81.7%,while in the other regions were 72.7%-86.2%.The phylogenetic tree of P2 region showed that 6 CV-A2 strains had a close evolutionary distance with the coxackievirus A4(CV-A4)and the phylogenetic tree of P3 region showed that 2011FJFZ027,2012FJQZ311 and 2020FJPTN040 had close evolutionary distance with coxackievirus A6(CV-A6),coxackieviru A14(CV-A14),coxackievirus A8(CV-A8),respectively.Recombination analysis showed that 2011FJFZ027,2012FJQZ311 and 2020FJPTN040 maybe evolved from recombinant CV-A6 strain(GenBank accession number:KR706309),CV-A14 strain(GenBank accession number:KP036482)and CV-A8 st
作者
朱颖
陈炜
何文祥
翁育伟
Zhu Ying;Chen Wei;He Wenxiang;Weng Yuwei(Fujian Provincial Center for Disease Control and Prevention,Fujian Provincial Key Laboratory of Zoonosis Research,Fuzhou 350012,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2022年第4期417-423,共7页
Chinese Journal of Experimental and Clinical Virology
基金
国家科技重大专项课题(2017ZX10104001)
福建省自然科学基金(2016J01350)
福建省科技创新平台建设项目(2019Y2001)
福建省卫生健康科技计划项目(2021GGB012)
福建省医学创新课题(2016-CX-14)。
关键词
手足口病
柯萨奇病毒A2型
系统进化分析
重组分析
Hand,foot and mouth disease
Coxsackievirus A2
Sequence analysis
Recombination analysis
