摘要
筛选活性物质含量高、生长速度快、对温度、光照和盐度等环境变化适应能力强的藻株对微藻产业的发展具有重要意义。本研究从内蒙古哈马太湖分离纯化出一株绿藻,基于形态学、18S rDNA和5.8S rDNA部分序列特征鉴定其为Dunaliella sp.。对分离藻株的培养基种类、温度和盐度等环境因子进行优化和研究。研究表明:分离藻株的适宜培养基为Zarrouk培养基。在温度25℃、盐度20、光强200μmol/(m^(2)·s)和初始pH=9.3的条件下,分离藻株的总脂占藻株干质量的24.83%,不饱和脂肪酸占总脂肪酸的45.79%,其中亚麻酸和亚油酸分别占总脂肪酸的32.48%和8.05%。本研究可为在高光和较高pH条件下生产多不饱和脂肪酸(特别是α-亚麻酸)提供优良的候选藻株。
The screening of algal strains with high content of active substances,fast growth rate and strong adaptability to environmental changes such as temperature and light is important for the development of microalgal industry.In this study,a green algal strain was isolated and purified from Hamatai Lake,Inner Mongolia.It was identified as Dunaliella sp.based on morphological characteristics and 18 S rDNA and 5.8 S rDNA partial sequences.The type of culture medium and environmental factors such as temperature,salinity,light intensity and initial pH were optimized.The results showed that the isolates were suitable for growth in Zarrouk medium,at 25 ℃ and salinity 20,under light intensity of 200 μmol/(m^(2)·s),and at an initial pH of 9.3.Under these conditions,the total lipid of the isolated algal strains was 24.83% of the dry weight,and the unsaturated fatty acids accounted for 45.79% of the total fatty acids,of which linolenic acid and linoleic acid accounted for 32.48% and 8.05% of the total fatty acids,respectively.Our findings provided an excellent candidate strain for the production of polyunsaturated fatty acids,especially α-linolenic acid,under high light and high pH conditions.
作者
陈仁霞
朱葆华
曹子豪
李赟
靳桂勇
潘克厚
Chen Renxia;Zhu Baohua;Cao Zihao;Li Yun;Jin Guiyong;Pan Kehou(The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qingdao 266003,China;Marine Fishery Science and Food Production Process Function Laboratory,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第10期59-67,共9页
Periodical of Ocean University of China
基金
国家自然科学基金项目(U1706212)资助。
关键词
杜氏藻
培养条件优化
生长
Α-亚麻酸
多不饱和脂肪酸
Dunaliella sp.
optimization of culture condition
growth
α-linolenic acid
polyunsaturated fatty acid
