摘要
为探究南海北部表层海水微生物的多样性及部分真菌代谢产物的活性,采取平板涂布稀释的方法对南海北部8个站位表层海水样品进行分离,基于16S rRNA和ITS序列测定对纯培养的微生物进行初步鉴定,共分离到162株细菌和43株真菌。其中162株细菌分属于4个门,5个纲,15个目,21个科,35个属的61个种,变形菌门和放线菌门为优势门;43株真菌分属于子囊菌门和担子菌门的5个纲,7个目,7个科,7个属的14个种,曲霉属为优势属。从分离得到的真菌中选取16株真菌进行培养,对其粗提物进行组蛋白去乙酰化酶抑制活性、抗肿瘤活性和抑菌活性筛选,结果表明:在100μg·mL^(-1)浓度下具有组蛋白去乙酰化酶抑制活性,抑制率在44%~54%之间;在60μg·mL^(-1)浓度下对人宫颈癌细胞的抑制率在2%~37%之间;在200μg·mL^(-1)浓度下对嗜水气单胞菌(Aeromonas hydrophila)和维氏气单胞菌(Aeromonas Vickers)两株指示菌未显示抑菌效果。研究结果表明,南海局部地区表层海水可培养微生物多样性高;对部分真菌的活性测试结果为后续筛选天然产物来源的海洋药物提供了一定的参考。
To survive in the complex ocean environment,marine microbes have evolved a variety of adaptation systems,including the production of specific biomolecules and unique bioactive compounds which were never found in terrestrial environments.Marine microorganisms with abundant species and unique metabolism are important sources of marine natural products and new medicines.The South China Sea(SCS)is the largest sea in China.The abundant microbial resources in SCS are ideal objects for studying marine microbial diversity and natural products.To study the microbial diversity of surface seawater in SCS and bioactivity of fungi extracts,a study was carried out.Surface seawater samples were collected from eight stations in the northern part of SCS,then were filtered and diluted to different concentrations and spread on bacterial and fungal plates,finally,the plates were cultured in a constant temperature incubator at 28℃ for 3-28 d.After cultivation,the morphological characteristics of the colonies were visually observed.Colonies of different forms were brought up in corresponding culture media after preliminary screening and purified into single colonies,then stored under 4℃ on an inclined plane.With a molecular protocol by DNA amplification and sequencing of the 16 S rRNA and ITS gene sequence alignment,a total of 162 bacterial strains and 43 fungal strains were isolated.The 162 bacterial strains belonged to 4 phyla,5 classes,15 orders,21 families,35 genera and 61 species.Proteobacteria and Actinomycetes were the dominant phylum.A total of 105 strains of Proteobacteria were identified as 84 strains of α-Proteobacteria,accounting for 51.8%;and 21 strains of γ-Proteobacteria,accounting for 12.9%;50 strains of Actinomycetes,accounting for 30.8%,4 strains of Bacteroidetes,accounting for 2.5%,3 strains of Firmicutes,accounting for 1.9%.Microbacterium and Erythrobacter were the dominant genera,which isolated 40 and 23 strains,respectively;E.pelagi was the highest with 12 strains.E.flavus,M.aurantiacum and M.schleiferi fol
作者
李涵
樊成奇
陈莎
周进
周俊芳
陆亚男
马丽艳
田晓清
LI Han;FAN Chengqi;CHEN Sha;ZHOU Jin;ZHOU Junfang;LU Yanan;MA Liyan;TIAN Xiaoqing(College of Food Science,Shanghai Ocean University,Shanghai 201306;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090;Key Laboratory of Oceanic and Polar Fisheries,Ministry of Agriculture and Rural Affairs,Shanghai 200090,China;Key Laboratory of East China Sea Fishery Resources Exploitation,Ministry of Agriculture and Rural Affairs,Shanghai200090,China;Key Laboratory of Inland Saline alkaline Aquaculture,Ministry of Agriculture and Rural Affairs,Shanghai 200090)
出处
《海洋渔业》
CSCD
北大核心
2022年第4期446-458,共13页
Marine Fisheries
基金
上海市科委扬帆计划(20YF1459800)
农业农村部财政项目(南海渔业资源调查与评估,NFZX2013)
中国水产科学研究院基本科研业务费资助项目(2020TD68)
中国水产科学研究院东海水产研基究所基本科研业务费(L32201924390)
上海市科研计划项目(18391901700)。
关键词
南海表层海水
微生物多样性
组蛋白去乙酰化酶抑制活性
抗肿瘤活性
South China Sea surface water
microorganism diversity
histone deacetylase inhibitory activity
anti-tumor activity
