摘要
为了根据遗传背景快速区分和鉴定甜菜品种的真实性和特异性,补充和打破形态学鉴定法的短板和局限,以96份甜菜登记品种为试材,采用DAMD分子标记技术构建了甜菜品种的分子身份证。结果显示,有7条扩增条带清晰、多态性较好的DAMD引物可用于鉴别甜菜品种,这7条引物共检测出103条带,其中多态性条带101条,多态性98.1%;96个品种间的最小遗传距离为0.039,最大为0.485,平均遗传距离0.237。通过聚类分析,96个甜菜品种被分为两类群,类群I仅有54号品种,类群II包含其余95个品种,类群II进一步又分两个亚群。仅利用URP1F、URP17R和URP2R这3条引物组合将96个供试甜菜品种完全区分。利用DAMD引物构建甜菜品种的分子身份证,为快速、高效地鉴定甜菜品种提供了一种新的途径,也为保护育种家和农民的利益提供了保障。
In order to quickly distinguish and identify the authenticity and specificity of sugar beet varieties according to their genetic backgrounds, and to replenish the shortcoming and break the limitation of morphological identification, using 96 registered sugar beet varieties as test materials, the molecular identity of varieties was constructed by DAMD molecular marker technology. The results showed that 7 DAMD primers with clear amplification bands and good polymorphism could be used to identify sugar beet varieties. A total of103 bands were detected, including 101 polymorphic bands, and the polymorphism was 98.1%. The minimum genetic distance among the 96 varieties was 0.039, the maximum was 0.485, and the average genetic distance was 0.237. Through cluster analysis, the 96 sugar beet varieties were divided into two groups, group I only had variety number 54, group II contained the rest 95 varieties, and group II was further divided into two subgroups. After that, the 96 sugar beet varieties were completely distinguished by the combination of three primers URP1F, URP17R and URP2R. The establishment of molecular identity of sugar beet varieties based on DAMD primers provided a new way for rapid and efficient identification of sugar beet varieties, and also provided a guarantee for protecting the interests of breeders and farmers.
作者
张紫若
马佳洁
高秋雨
吴则东
ZHANG Ziruo;MA Jiajie;GAO Qiuyu;WU Zedong(College of Advanced Agriculure and Ecological Environment Department,Heilongjiang University,Harbin 150080;Heilongjang Provincial Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University,Harbin 150080)
出处
《中国农学通报》
2022年第23期21-26,共6页
Chinese Agricultural Science Bulletin
基金
财政部和农业农村部“国家现代农业产业技术体系资助”(CARS-170111)。
关键词
甜菜
DAMD
分子标记
分子身份证
指纹图谱
聚类分析
sugar beet
DAMD
molecular marker
molecular identity
fingerprint
clustering analysis
