摘要
采用超声辅助响应面法对花生中黄曲霉毒素(Aflatoxin B1/B2/G1/G2,AFTS)的提取工艺进行优化,得到最佳提取工艺为65%甲醇溶液、超声时间15 min、超声功率206 W、料液比1∶4(g∶mL),利用该工艺,AFTS的提取量可达18.06μg/kg.在此最优工艺条件下提取AFTS,并结合高效液相色谱(HPLC)—光化学柱后衍生法作为参考方法,对市场上主流的8家黄曲霉毒素B1定性试剂盒及2家黄曲霉毒素B1定量试剂盒进行质量评价方法的验证,结果表明定性试剂盒检测结果灵敏度为100%,特异性98%,假阴性率0,假阳性率2%;定量试剂盒准确度及精密度较高,线性拟合度均>0.99,可满足检测需求.
The extraction process of aflatoxin(AflatoxinB1/B2/G1/G2,AFTS)from peanuts is optimized by ultrasound-assisted response surface method.The optimum extraction process is obtained as follows:65%methanol solution,ultrasonic time 15 min,ultrasonic power 206 W,and the ratio of material to liquid 1∶4.Under this optimal condition,the extraction of AFTS is 18.06μg/kg.The peanut samples are pretreated with the best technology and detected by high performance liquid chromatography(HPLC)with photochemical post-column derivation method,which is used as a reference method to evaluate the quality of the rapid detection kit.There are eight quantitative quits and two qualitative quits populared in the market to be evaluated and tested.The results show that the sensitivity of the qualitative kit is 100%,that the specificity is 98%,that the false negative rate is 0,and that the false positive rate is 2%.The accuracy and precision of the quantitative kit are high,and the linear fitting degree is more than 0.99,which can meet the detection requirements.
作者
赵萍
张盈月
周家宏
陈飞
Zhao Ping;Zhang Yingyue;Zhou Jiahong;Chen Fei(School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China;Nanjing Engineering Research Center for Functional Components Development of Featured Biological Resources,Nanjing Normal University,Nanjing 210023,China;School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
出处
《南京师范大学学报（工程技术版）》
CAS
2022年第3期72-82,共11页
Journal of Nanjing Normal University(Engineering and Technology Edition)
基金
“十三五”国家重点研发计划重点专项项目(2018YFC1603400)。
关键词
响应面设计
黄曲霉毒素
快检试剂盒
评价验证
response surface design
aflatoxin
quick detection kit
evaluation of validation
