摘要
目的:研究胰腺癌患者血浆和癌组织中microRNA(miRNA)-10和张力蛋白同源物基因(PTEN)的表达,并探讨两者在胰腺癌中的调控关系;研究miRNA-10在人胰腺癌PANC-1细胞中的表达及其对PANC-1细胞增殖和迁移的影响。方法:选择80例胰腺癌患者为研究组及80例健康体检者为对照组。采用实时荧光定量PCR技术检测胰腺癌患者血浆中miRNA-10和PTEN基因及组织中miRNA-10的表达,并分析miRNA-10和PTEN基因表达的相关性。采用四甲基偶氮唑盐法和细胞集落形成实验研究miRNA-10在人胰腺癌PANC-1细胞生长和增殖能力,采用划痕实验检测细胞的迁移能力。结果:两组血浆中miRNA-10和PTEN mRNA的表达水平比较,差异均有统计学意义(P<0.05)。miRNA-10和PTEN mRNA在胰腺癌患者血浆中表达呈线性负相关(r=-0.716,P<0.01)。miRNA-10在胰腺癌患者血浆和组织中表达呈正相关(r=0.938,P<0.01)。MTT法检测结果显示,miRNA-10细胞转染24、48、72、96 h的A值均高于对照组(P<0.05)。平板克隆形成实验检测结果显示:miRNA-10转染2周后,miRNA-10转染的细胞克隆数均高于对照组(P<0.05)。划痕实验结果显示:转染miRNA-10分别在24、48 h的PANC-1细胞中,miRNA-10划痕间距与对照组比较,差异均有统计学意义(P<0.05);PTEN组与对照组比较,差异均无统计学意义(P>0.05)。结论:PTEN可能是miRNA-10的作用靶点,miRNA-10通过负性调控PTEN基因参与胰腺癌的发生发展、侵袭和转移,miRNA-10可作为胰腺癌的辅助诊断的一个新的检测指标。
Objective:To investigate the expression of microRNA(miRNA)-10 and tension protein homologue(PTEN)in plasma and cancer tissues of patients with pancreatic cancer and to explore their relationship with pancreatic cancer.To study the expression of miRNA-10 in human pancreatic cancer PANC-1 cells and its effect on proliferation and migration of PANC-1 cells.Method:A total of 80 patients with pancreatic cancer were selected as the study group and 80 healthy subjects as the control group.Real time fluorescence quantitative PCR(qRT-PCR,qRT-PCR)was used to detect the expression of miRNA-10 and PTEN genes and miRNA-10 in tissues of patients with pancreatic cancer,and the correlation between miRNA-10 and PTEN gene expression was analyzed.The growth and proliferation ability of miRNA-10 in human pancreatic cancer PANC-1 cells was studied by four methazolium salt method and colony forming assay,the migration ability of cells was detected by scratch test.Result:There were significant differences in the expression levels of miRNA-10 and PTEN mRNA in plasma between the two groups(P<0.05).There was a linear negative correlation between the expression of miRNA-10 and PTEN mRNA in the plasma of patients with pancreatic cancer(r=-0.716,P<0.01).The expression of miRNA-10 in plasma and tissues of patients with pancreatic cancer was positively correlated(r=0.938,P<0.01).MTT assay showed that the A value of miRNA-10 cells transfected for 24,48,72 and 96 h were higher than those of the control group(P<0.05).The results of plate clone formation test showed that two weeks after miRNA-10 transfection,the number of cell clones transfected with miRNA-10 was higher than that of the control group(P<0.05).The results of scratch test showed that there were significant differences in the scratch spacing of transfected miRNA-10 in PANC-1 cells at 24 and 48 h compared with the control group(P<0.05);There were no significant differences between PTEN group and control group(P>0.05).Conclusion:PTEN may be the target of miRNA-10,miRNA-10 participates i
作者
刘玉兰
何凤屏
郭伟强
刘英
黄从云
李定云
黄亮
陈晓旋
LIU Yulan;HE Fengping;GUO Weiqiang;LIU Ying;HUANG Congyun;LIDingyun;HUANG Liang;CHEN Xiaoxuan(Affiliated Yuebei People’s Hospital,Shantou University Medical College,Shaoguan 512025,China;不详)
出处
《中国医学创新》
CAS
2022年第25期29-34,共6页
Medical Innovation of China
基金
广东省生物医学创新平台建设项目(粤卫函[2018]1254号)。
