摘要
抗菌药物耐药已成为棘手的全球性健康问题。近年来,基于宏基因组下一代测序(metagenomic next-generation sequencing,mNGS)的病原体检测技术,因其不受培养限制、时效性高、病原体覆盖广的特点,已广泛应用于临床诊断。该技术在药物敏感性检测方面的应用价值也备受关注。该文总结和归纳了基于mNGS的抗菌药物敏感性检测方法,从耐药基因数据库、序列比对软件、耐药基因数据比对方法和耐药预测模型方面分析比较了mNGS药物敏感性检测的分析流程,并探讨了mNGS耐药分析临床应用存在的问题。
Antimicrobial resistance is a rigorous health issue around the world.Because of the short turn-aroundtime and broad pathogen spectrum,culture-independent metagenomic next-generation sequencing(mNGS)is a powerful and highly efficient tool for clinical pathogen detection.The increasing question is whether mNGS is practical in the prediction of antimicrobial susceptibility.This review summarizes the current mNGS-based antimicrobial susceptibility testing technologies.The critical determinants of mNGS-based antibacterial resistance prediction have been comprehensively analyzed,including antimicrobial resistance databases,sequence alignment tools,detection tools for genomic antimicrobial resistance determinants,as well as resistance prediction models.The clinical challenges for mNGS-based antibacterial resistance prediction have also been reviewed and discussed.
作者
王婧
陈勃江
周永召
李为民
WANG Jing;CHEN Bojiang;ZHOU Yongzhao;LI Weimin(Precision Medicine Center,Precision Medicine Key Laboratory of Sichuan Province,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China;Department of Respiratory and Critical Care Medicine,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China)
出处
《华西医学》
CAS
2022年第8期1121-1127,共7页
West China Medical Journal
基金
四川省科技计划项目(2020YFS0573)。
关键词
宏基因组下一代测序
药物敏感性检测
病原体
耐药性
Metagenomic next-generation sequencing
antimicrobial susceptibility testing
pathogen
antimicrobial resistance
