摘要
以2个对高温及淹水具不同耐性的不结球白菜品种‘黑油冬’、‘苏州青’为材料,设置24℃、35℃、40℃3个温度梯度,每个温度下设置淹水和不淹水2个处理,研究高温和淹水胁迫对不结球白菜纤维素合成相关酶活性的影响。结果表明,在高温胁迫下,‘黑油冬’、‘苏州青’的CesA、SS、KOR酶活性呈先上升后下降的变化趋势,而CE酶活性的变化趋势与此相反。在高温和淹水共同胁迫下,‘黑油冬’、‘苏州青’的纤维素合成相关酶活性变化趋势与单一高温胁迫相似,只是受影响的程度大于单一高温胁迫。此外,无论是高温胁迫,还是高温和淹水共同胁迫,‘黑油冬’体内CesA、SS、KOR活性上升的幅度大于‘苏州青’,而下降的幅度却小于‘苏州青’;‘黑油冬’的CE活性上升的幅度小于‘苏州青’,而下降的幅度却大于‘苏州青’。
Two non-heading Chinese cabbage varieties‘Heiyoudong’and‘Suzhouqing’were used as materials to study the effects of high temperature and waterlogging stress on the activities of enzymes related to cellulose synthesis in non-heading Chinese cabbage.The two varieties had different resistance to high temperature and waterlogging.Three temperature gradients of 24℃,35℃ and 40℃ were set,and each temperature was set two treatments,waterlogging and non-waterlogging.The results showed that under high temperature stress,the CesA,SS and KOR enzyme activities of‘Heiyoudong’and‘Suzhouqing’showed a trend of first increasing and then decreasing,while the trend of CE enzyme activity was opposite.Under the combined stress of high temperature and waterlogging,the changed trend of the enzyme activities related to cellulose synthesis in‘Heiyoudong’and‘Suzhouqing’was similar to that of a single high temperature stress,but the degree of impact was greater than that of a single high temperature stress.In addition,regardless of high temperature stress,high temperature and waterlogging combined stress,the increase in CesA,SS and KOR activities in‘Heiyoudong’was greater than that of‘Suzhouqing’,but the decrease was less than that of‘Suzhouqing’;the increase in CE activity of‘Suzhouqing’was less than that of‘Suzhouqing’,but the decrease rate was greater than that of‘Suzhouqing’.
作者
朱红芳
高璐
何小艳
李晓锋
奚丹丹
朱玉英
Zhu Hongfang;Gao Lu;He Xiaoyan;Li Xiaofeng;Xi Dandan;Zhu Yuying(Horticulture Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403)
出处
《分子植物育种》
CAS
北大核心
2022年第15期5107-5114,共8页
Molecular Plant Breeding
基金
上海市农委科技兴农技术创新项目(沪农科创字[2020]第1-4号)资助。
关键词
不结球白菜
高温
淹水
纤维素酶
Non-heading Chinese cabbage
High temperature
Waterlogging
Cellulase enzyme
