摘要
本研究以1年生、2年生和3年生的白芨根为研究材料,通过BGISEQ-500高通量测序平台进行转录组测序,测序结果共获得76552条Unigenes序列。与七大数据库相似性比对结果显示,得到注释的Unigenes共有52219条,占所有Unigenes的68.12%。对3个不同年限的白芨根转录组数据进行比较分析,共发现37825条差异基因,涉及了47种生物功能,可划分为分子功能、细胞组分和生物学过程三大类。通过KEGG富集分析发现共涉及134条信号通路,主要的信号通路为代谢通路和次生代谢产物合成通路。共有59条关键酶差异基因参与了甘露糖的合成途径,多条途径参与了葡萄糖的合成;12条根膨大相关的差异基因参与了白芨根的膨大生长调控。RT-qPCR分析验证了与根膨大和糖代谢相关的6个具有代表性的基因表达与转录组结果趋于一致。本研究结果为进一步深入研究白芨根生长发育和活性成分的生物合成的分子调控机制提供理论依据。
In this study,one-,two-and three-year old Bletilla striata roots were used for transcriptional sequencing by the BGISEq-500 high-throughput sequencing platform.A total of 76552 unigene sequences were available from the sequencing data.The results of similarity comparison with the seven major databases show that a total of 52219 Unigenes have been annotated,accounting for 68.12% of all Unigenes.The transcriptional data of B.striata roots during three growth periods were compared and analyzed.A total of 37825 differential gene were found,involving 47 biological functions,which were divided into three categories:biological process,cellular component and molecular function.Through KEGG enrichment analysis,it was found that 134 signal pathways were involved.The main signal pathway was the metabolic pathway,followed by the secondary metabolite synthesis pathway.In addition,there are a total of 59 key enzyme differential genes involved in the synthesis of mannose;multiple pathways are involved in the synthesis of glucose;a total of 12 differential genes related to root expansion are involved in the expansion and growth of B.striata roots.The results of RT-qPCR analysis of 6representative genes related to root enlargement and sugar metabolism are basically consistent with the transcriptome results.The research results provide theoretical basis for further research on the molecular regulation mechanism of B.striata root growth and biosynthesis of active ingredients.
作者
齐家森
吴远双
李一果
徐慧妮
李昆志
Qi Jiasen;Wu Yuanshuang;Li Yiguo;Xu Huini;Li Kunzhi(College of Life Science and Technology,Kunming University of Science and Technology,Kunming,650000)
出处
《分子植物育种》
CAS
北大核心
2022年第15期4990-4999,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760349,31960071)资助。
