摘要
背景:髌腱是维持膝关节稳定的重要结构,在拉伸作用下表现出特有的非线性力学特性,但是弹性蛋白在拉伸载荷下对髌腱力学行为的影响尚不清楚。目的:通过弹性蛋白的靶向酶处理,研究弹性蛋白对髌腱力学性能的影响。方法:(1)将30个40-50 mg新鲜猪髌腱平均分配到5种不同弹性蛋白酶浓度(0,1,5,10,20 U/m L)中孵育以及在5个不同的时间(0,1,4,6,12h)的弹性蛋白酶溶液中孵育,最后检测弹性蛋白含量。(2)将髌腱分别在PBS溶液、5 U/m L弹性蛋白酶溶液中处理8h,进行Verhoeff VanGieson(VVG)染色和Masson染色。(3)将20个新鲜猪髌腱随机分为PBS对照组和弹性蛋白酶处理组(弹性蛋白酶组),在试样表面标记9个点后进行纵向拉伸及应力松弛实验,使用光学非接触法计算标记点的位移用于后续应变分析并计算应力;然后将试样分别在PBS溶液、5 U/m L弹性蛋白酶溶液中孵育8 h,再次进行同样的力学测试。结果与结论:(1)在5 U/m L弹性蛋白酶溶液中孵育8 h可以满足此次实验要求;(2)与处理前相比,PBS组和酶处理组的组织拉伸应力显著降低,均降低约40%(P<0.001,P<0.01);与处理前相比,处理后的松弛百分比显著增加,前后相差约12%(P<0.05);在归一化的平均应力-时间曲线上,酶处理组比PBS组应力降低更大,两者相差约16%(P<0.0001);(3)以上结果表明,弹性蛋白在髌腱的拉伸力学特性及黏弹性特性中起到重要作用,进一步补充了对多尺度肌腱结构-功能关系的理解,这有利于未来对微观结构本构模型的发展和改进,以模拟髌腱病变及手术干预。
BACKGROUND:Patellar tendon is an important structure to maintain the stability of the knee joint and exhibits unique nonlinear mechanical properties under tension,but the effect of elastin on the mechanical behavior of the patellar tendon under tensile loading is still unclear.OBJECTIVE:To study the effect of elastin on the mechanical properties of patellar tendon through targeted enzyme treatment of elastin.METHODS:(1)Thirty fresh porcine patellar tendons,weighing 40–50 mg,were evenly divided into five different elastase concentrations(0,1,5,10,20 U/mL)or incubated in elastase solutions for five different times(0,1,4,6,12 hours),and finally the elastin content was detected.(2)Patellar tendon was treated in PBS or 5 U/mL elastase for 8 hours,then Verhoeff Van Gieson(VVG)staining and Masson staining were performed.(3)Twenty fresh porcine patellar tendons were randomly divided into PBS control group and elastase-treated group.After 9 points were marked on the sample surface,the longitudinal tensile test and stress relaxation test were carried out.The displacement of the marked points was calculated by optical non-contact method for subsequent strain analysis and stress calculation.The samples were incubated in PBS or 5 U/mL elastase for 8 hours,and the same mechanical test was performed again.RESULTS AND CONCLUSION:(1)Incubating in 5 U/mL elastase for 8 hours could meet the requirements of this experiment.(2)Compared with pre-treatment,tissue tensile stress was significantly reduced in the PBS and elastase-treated groups,which were both decreased by about 40%(P<0.001,P<0.01).Compared with pre-treatment,the relaxation percentage after treatment increased significantly,with a difference of about 12%(P<0.05).On the normalized average stress-time curve,the stress of elastase-treated treatment group decreased more than that of PBS group,with a difference of about 16%(P<0.0001).(3)The above results showed that elastin played an important role in the tensile mechanical properties and viscoelastic properties of patellar
作者
刘晓云
邓羽平
李飞飞
赵东良
杨洋
黄涛
谭文长
吴耀彬
黄文华
李严兵
Liu Xiaoyun;Deng Yuping;Li Feifei;Zhao Dongliang;Yang Yang;Huang Tao;Tan Wenchang;Wu Yaobin;Huang Wenhua;Li Yanbing(Guangdong Engineering Research Center for Translation of Medical 3D Printing Application,Guangdong Provincial Key Laboratory of Medical Biomechanics,National Key Discipline of Human Anatomy,School of Basic Medical Sciences,Southern Medical University,Guangzhou 510515,Guangdong Province,China;Department of Orthopedics and Traumatology,Integrated Hospital of Traditional Chinese Medicine and Western Medicine,Southern Medical University,Guangzhou 510315,Guangdong Province,China;Institute of Biomedical Engineering,Shenzhen Bay Laboratory,Shenzhen 518132,Guangdong Province,China;School of Chemical Biology and Biotechnology,Peking University Shenzhen Graduate School,Shenzhen 518055,Guangdong Province,China;The Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510378,Guangdong Province,China;Guangdong Medical Innovation Platform for Translation of 3D Printing Application,The Third Affiliated Hospital of Southern Medical University,Guangzhou 510000,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第18期2831-2836,共6页
Chinese Journal of Tissue Engineering Research
基金
广东省科技计划项目(2018B090944002),项目负责人:李严兵
广东省基础与应用基础研究基金(2020B1515120001),项目负责人:黄文华
深圳市科技计划(JCYJ20210324130401005),项目负责人:赵东良。
关键词
髌腱
生物力学
应力松弛
弹性蛋白
准静态拉伸
弹性蛋白酶
黏弹性
酶处理
patellar tendon
biomechanics
stress relaxation
elastin
quasi-static tensile
elastase
viscoelasticity
enzyme treatment
