摘要
目的 探讨羊栖菜多糖(SFPS)对3T3-L1前脂肪细胞增殖及凋亡的作用及其机制。方法 采用MTT法测定不同浓度SFPS对3T3-L1前脂肪细胞增殖活性的影响,应用Hoechst33258染色法检测细胞凋亡情况,实时荧光定量逆转录-聚合酶链反应(RT-PCR)检测Bax、Bcl-2和Caspase-3基因m RNA表达量的变化。结果 与对照组比较,不同浓度SFPS可降低3T3-L1前脂肪细胞增殖活力,诱导细胞凋亡,明显降低Bcl-2基因m RNA表达(P<0.05),明显增高Bax和Caspase-3基因m RNA表达(P<0.05)。结论 SFPS能够抑制3T3-L1前脂肪细胞增殖活性,其作用机制可能与诱导细胞凋亡,调控相关基因Bcl-2、Bax和Caspase-3 mRNA的表达有关。
Objective To explore the effect and mechanism of SFPS on 3T3-L1 preadipocytes proliferation and apoptosis.Methods MTT assay was used to determine the effect of different concentrations of SFPs on the proliferation of 3T3-L1preadipocytes.Hoechst33258 staining was used to detect the apoptosis.Real time fluorescence quantitative reverse transcription polymerase chain reaction(RT-PCR) was used to detect the changes of Bax,Bcl-2 and caspase-3 gene mRNA expression.Results Different concentration of SFPS could inhibite proliferation of 3T3-L1 preadipocytes,and induce apoptosis of 3T3-L1preadipocytes.SFPS could effectively reduce the level of Bcl-2 gene mRNA expression and improve the level of Caspase-3 and Bax gene mRNA expression.Conclusion SFPS could inhibit proliferation of 3T3-L1 preadipocytes,and the mechanism might be that SFPS significantly induced apoptosis through regulating Bax、Bcl-2 and Caspase-3 gene expression.
作者
杨旭东
石杰
杨骄霞
王桂云
宋高臣
张杰
YANG Xu-Dong;SHI Jie;YANG Jiao-Xia;WANG Gui-Yun;SONG Gao-Chen;ZHANG Jie(Department of Pharmacology,Mudanjiang Medical College,Mudanjiang 157011,China;Department of Cardiology,HongQi Hospital Affiliated to Mudanjiang Medical College,Mudanjiang 157011,China;Department of Biochemistry,Mudanjiang Medical College,Mudanjiang 157011,China)
出处
《中国药物经济学》
2022年第7期101-104,共4页
China Journal of Pharmaceutical Economics
基金
黑龙江省省属高等学校基本科研业务费科研项目(2018-KYYWFMY-0035)。
关键词
羊栖菜多糖
3T3-L1前细胞
凋亡
基因表达
Sargassum fusiforme polysaccharide
3T3-L1 preadipocytes
Apoptosis
Gene expression
