摘要
为明确引起贵州梨芽枯病的间座壳菌种类,本研究进行了病害调查、病芽采集和病菌分离及纯化,根据其菌落特征与ITS序列鉴定,共获得94个间座壳属Diaporthe菌株,从中选取40个代表菌株进行多基因(ITS、TEF、CAL、HIS和TUB)系统发育分析和形态学观察,结果显示它们分属于间座壳属的8个种,包括核桃间座壳菌D.biguttulata、榆树间座壳菌D.eres、椭圆间座壳菌D.ellipsoidea、梭状间座壳菌D.fusicola、香港间座壳菌D.hongkongensis、塞纳间座壳菌D.sennae、大豆间座壳菌D.sojae和蜜柑间座壳菌D.unshiuensis,其中D.ellipsoidea、D.biguttulata和D.sennae为侵染梨的首次报道。玉露香梨离体枝条接种结果显示,8种间座壳的代表菌株均可产生与田间相同的症状,分离比最高的D.eres致病力较强,表明它们均为梨芽枯病的病原菌,其中D.eres为病原优势种。本研究首次报道了引起梨芽枯的间座壳菌种类组成,为该病的发生规律及防控技术研究提供了理论依据。
Disease investigation,collection of diseased buds,isolation and purification of pathogens were conducted to clarify the species of Diaporthe causing pear bud witherings in Guizhou.In total,94 Diaporthe isolates were obtained based on their colony characteristics combined with ITS sequence identification,of which 40 representative isolates were chosen for carrying out multi-gene(ITS,TEF,CAL,HIS and TUB)phylogenetic analysis and morphological observation.Eight species of Diaporthe were identified,including D.biguttulata,D.eres,D.ellipsoidea,D.fusicola,D.hongkongensis,D.sennae,D.sojae and D.unshiuensis.To our knowledge,D.ellipsoidea,D.biguttulata and D.sennae were first reported to infect pears.Inoculating in vitro branch of“Yuluxiang”pear showed that the eight species of Diaporthe could produce symptoms the same as those in the field,indicating that they were the pathogens causing pear bud witherings.D.eres with the highest isolation rate was dominant and had stronger pathogenicity.This is the first report of Diaporthe species causing pear bud witherings,providing useful data for practicable disease control of pear in Guizhou Province.
作者
王先洪
姜佳琦
洪霓
王国平
WANG Xianhong;JIANG Jiaqi;HONG Ni;WANG Guoping(Hubei Crop Disease Monitoring&Safety Control Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第8期1151-1164,共14页
Mycosystema
基金
财政部和农业农村部国家现代农业产业技术体系资助(CARS-28)。
