摘要
为明确青皮核桃表面的真菌多样性特征,以‘绿岭一号’青皮核桃为试材,利用高通量测序法分析‘绿岭一号’青皮核桃表面真菌群落组成及其多样性,并采用FUNGuild数据库预测真菌群落生态功能。结果表明,青皮核桃表面的真菌一共有4个门、14个纲、12个目、18个科和18个属。子囊菌门(Ascomycota)是青皮核桃表面真菌的主要真菌门类,相对丰度为97%,主要属类为葡萄座腔菌属(Botryosphaeria)、炭疽菌属(Colletotrichum)、有孢汉逊酵母属(Hanseniaspora)、链格孢霉属(Alternaria)、螺旋聚孢霉属(Clonostachys)、间座壳菌属(Diaporthe)、镰刀菌属(Fusarium)。真菌FUNGuild功能预测结果表明:青皮核桃采后主要以病理营养型真菌为主,其次为腐生营养型,其占比分别为28.42%和18.94%。使用高通量测序技术确定了青皮核桃采后腐烂病病原菌的主要类群,为青皮核桃腐烂病的防治提供理论依据。
In order to clarify the characteristics of fungal diversity on the surface of green walnuts,the composition of fungal communities and their diversity on the surface of green walnuts were analyzed by high-throughput sequencing using‘Green Ridge No.1’green walnuts as test material.FUNGuild database was used to predict the ecological functions of fungal communities.The results showed that,there were 4 phyla,14 classes,12 orders,18 families and 18 genera of fungi on the surface of green walnuts.Ascomycota was the main fungal phylum on the surface of green walnut,with a relative abundance of 97%,and the main genera were Botryosphaeria,Colletotrichum,Hanseniaspora,Alternaria,Clonostachys,Diaporthe,and Fusarium.Fungi FUNGuild function prediction results showed that there were mainly pathotrophic fungi(pathotroph),followed by saprophytic nutrient type on the green walnuts after harvest,accounted for 28.42%and 18.94%,respectively.High-throughput sequencing technology was used to identify the main group of green walnut rot pathogens,which provides a theoretical basis for the prevention and treatment of green walnut rot disease.
作者
张琴
康明丽
赵茹
宋丽君
集贤
何爱民
吉洋洋
ZHANG Qin;KANG Mingli;ZHAO Ru;SONG Lijun;JI Xian;HE Aimin;JI Yangyang(School of Food and Biology,Hebei University of Science and Technology,Shijiazhuang 050000,China;Institute of Agricultural Products Preservation and Processing Technology,Tianjin Academy of Agricultural Sciences(National Agricultural Products Preservation Engineering Technology Research Center(Tianjin)),Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Ministry of Agriculture and Rural Affairs,Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Tianjin 300384,China;Hebei Lüling Kangwei Food Co.,Ltd.,Xingtai 054300,China)
出处
《保鲜与加工》
CAS
北大核心
2024年第8期102-108,共7页
Storage and Process
基金
河北省重点研发计划项目(21327101D)。
