摘要
目的分析脂联素(ADPN)、单核细胞趋化蛋白-1(MCP-1)、miRNA-103在老年反复发作性尿路感染患者中的表达及临床意义。方法选取2019年1月至2020年12月该院收治的112例首发老年尿路感染患者作为首发组,134例反复发作性尿路感染患者作为反复发作组,同期选取80例体检健康者作为对照组。采用酶联免疫吸附试验(ELISA)测定各组血清ADPN、MCP-1表达水平,采用实时荧光定量PCR法测定外周血单个核细胞miRNA-103表达水平。分析3组患者ADPN、MCP-1、miRNA-103表达水平,以及上述指标对老年反复发作性尿路感染的诊断价值。结果反复发作组ADPN水平低于首发组和对照组,MCP-1、miRNA-103高于首发组和对照组,差异均有统计学意义(P<0.05)。随着患者疾病严重程度加重,ADPN表达逐渐降低,MCP-1、miRNA-103表达逐渐升高,差异均有统计学意义(P<0.05)。预后不良患者ADPN表达低于预后良好患者,MCP-1、miRNA-103表达高于预后良好患者(P<0.05)。相关性分析显示,ADPN与MCP-1呈负相关(r=-0.543,P=0.001),ADPN与miRNA-103呈负相关(r=-0.552,P=0.001),MCP-1与miRNA-103呈正相关(r=0.649,P=0.001)。受试者工作特征(ROC)曲线分析显示,ADPN、MCP-1、miRNA-103单独诊断老年反复发作性尿路感染的截断值分别为10.00μg/mL、65.98 pg/mL、0.82,三项联合对反复发作性尿路感染的诊断价值高于单项检测(P<0.05)。结论老年反复发作性尿路感染病ADPN表达降低,MCP-1、miRNA-103表达升高,三者表达水平与患者疾病严重程度及预后相关。
Objective To analyze the expression and clinical significance of adiponectin(ADPN),monocyte chemoattractant protein-1(MCP-1)and miRNA-103 in elderly patients with recurrent urinary tract infection.Methods A total of 112 first-episode elderly patients with urinary tract infection admitted to this hospital from January 2019 to December 2020 were selected as the first-episode group,134 patients with recurrent urinary tract infection were selected as the recurrent group,and 80 healthy subjects were selected as the control group during the same period.The expression levels of ADPN and MCP-1 were determined by enzyme-linked immunosorbent assay(ELISA),and miRNA-103 in peripheral blood mononuclear cells were determined by real-time fluorescence quantitative PCR.The expression levels of ADPN,MCP-1 and miRNA-103 in the three groups were analyzed,as well as the diagnostic value of the above indicators for elderly patients with recurrent urinary tract infection.Results The level of ADPN in recurrent group was lower than that in first-episode group and control group,while MCP-1 and miRNA-103 were higher than those in first-episode group and control group,with statistical significance(P<0.05).With the aggravation of disease severity,the expression of ADPN gradually decreased,while the expression of MCP-1 and miRNA-103 gradually increased,with statistical significance(P<0.05).The expression of ADPN in patients with poor prognosis was lower than that in patients with good prognosis,and the expression of MCP-1 and miRNA-103 were higher than those in patients with good prognosis(P<0.05).Correlation analysis showed that ADPN negatively correlated with MCP-1(r=-0.543,P=0.001),ADPN negatively correlated with miRNA-103(r=-0.552,P=0.001),and MCP-1 positively correlated with miRNA-103(r=0.649,P=0.001).Receiver operating characteristic(ROC)curve analysis showed that the cut-off values of ADPN,MCP-1 and miRNA-103 were 10.00μg/mL,65.98 pg/mL and 0.82 in the diagnosis of recurrent urinary tract infection in the elderly,and the combined dia
作者
侯桂平
郑雪君
李润奇
崔利
杜玄
李华
孙建宇
HOU Guiping;ZHENG Xuejun;LI Runqi;CUI Li;DU Xuan;LI Hua;SUN Jianyu(Department of Clinical Laboratory,the Second Hospital of Zhangjiakou,Zhangjiakou,Hebei 075000,China;Department of Orthopedics,the Second Hospital of Zhangjiakou,Zhangjiakou,Hebei 075000,China;Department ofUrology,the Second Hospital of Zhangjiakou,Zhangjiakou,Hebei 075000,China)
出处
《国际检验医学杂志》
CAS
2022年第16期1954-1958,共5页
International Journal of Laboratory Medicine
基金
河北省中医药管理局2021年度中医药类科研计划(2021442)。
