摘要
目的探讨微小RNA-187(miR-187)、基质金属蛋白酶2(MMP2)、癌胚抗原(CEA)在胆囊癌患者中的表达水平及临床意义。方法采用回顾性研究方法,收集2016年1月至2018年6月西安高新医院肿瘤科收治的胆囊癌患者102例作为癌症组,100例胆囊良性肿瘤患者作为良性组,收集同期100例体检健康者作为对照组,比较3组研究对象miR-187、MMP2、CEA水平。采用受试者工作特征曲线(ROC曲线)分析miR-187、MMP2、CEA对胆囊癌的诊断效能。采用COX回归分析胆囊癌患者预后的影响因素。结果癌症组miR-187、MMP2、CEA水平明显高于对照组及良性组,良性组miR-187、MMP2、CEA水平明显高于对照组,差异有统计学意义(P<0.05)。ROC曲线分析结果显示,miR-187、MMP2、CEA联合检测诊断胆囊癌的曲线下面积为0.908,灵敏度和特异度分别为92.2%和80.0%。COX回归分析结果显示,Nevin分期、TNM分期、分化程度、淋巴结转移情况及miR-187、MMP2、CEA水平是胆囊癌患者预后的独立影响因素(P<0.05)。结论miR-187、MMP2、CEA水平在胆囊癌患者中明显升高,是影响患者预后的因素,且3项指标联合检测有助于胆囊癌的早期诊断。
Objective To investigate the expression levels and clinical significance of microRNA-187(miR-187),matrix metalloproteinase 2(MMP2)and carcinoembryonic antigen(CEA)in patients with gallbladder cancer.Methods Used retrospective research method,a total of 102 patients with gallbladder cancer who were admitted to the Department of Oncology of Xi′an High-tech Hospital from January 2016 to June 2018 were collected as the cancer group,100 patients with benign gallbladder tumors were collected as the benign group,and 100 healthy subjects with physical examination during the same period were collected as the control group.The levels of miR-187,MMP2 and CEA were compared among the three groups of subjects.The receiver operating characteristic curve(ROC curve)was used to analyze the diagnostic performance of miR-187,MMP2 and CEA for gallbladder cancer.Cox regression analysis was used to analyze the prognostic factors of patients with gallbladder cancer.Results The levels of miR-187,MMP2 and CEA in the cancer group were significantly higher than those in the control group and benign group,and the levels of miR-187,MMP2 and CEA in the benign group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The results of ROC curve analysis showed that the area under the curve of the combined detection of miR-187,MMP2 and CEA for the diagnosis of gallbladder cancer was 0.908,and the sensitivity and specificity were 92.2%and 80.0%respectively.COX regression analysis showed that Nevin stage,TNM stage,degree of differentiation,lymph node metastasis,and miR-187,MMP2,CEA levels were independent prognostic factors in patients with gallbladder cancer(P<0.05).Conclusion The levels of miR-187,MMP2 and CEA are significantly increased in patients with gallbladder cancer,which are factors affecting the prognosis of patients,and the combined detection of the three indicators is helpful for the early diagnosis of gallbladder cancer.
作者
杨阳
贾万理
晋婕
YANG Yang;JIA Wanli;JIN Jie(Department of Blood Transfusion,Xi′an High-tech Hospital,Xi′an,Shaanxi 710075,China;Xi′an Jinyu Medical Laboratory Co.,Ltd,Xi′an,Shaanxi 710018,China)
出处
《检验医学与临床》
CAS
2022年第16期2239-2241,2245,共4页
Laboratory Medicine and Clinic
