摘要
针对污水处理厂深度脱氮需求,选用黄铁矿(FeS_(2))和聚羟基丁酸戊酸酯(PHBV)两种材料,构建应用于污水处理厂二级出水深度脱氮的反硝化生物填充床,通过进出水水质监测、材料结构表征(扫描电镜)、高通量测序等技术手段,探究了FeS_(2)-PHBV协同自养-异养反硝化体系的工艺运行效果及机理.结果表明,FeS_(2)-PHBV协同反硝化体系脱氮速率优于单独FeS_(2)自养体系,当水力停留时间为2h时,脱氮效率为90%以上,且产生更少的硫酸盐副产物.相比于单独PHBV异养体系,协同体系在达到相同总氮去除效果的同时释放更低的DOC(23.62mg/L).FeS_(2)-PHBV协同体系中两种材料表面结构均较利用前发生了显著变化,这是由于材料表面生物膜的富集和利用.共混反应器内优势菌群为厌氧发酵菌属(Sporomusaceae)和梭菌属(Clostridium sensu stricto 7),两者实现了共混体系异养-自养的协同反硝化效果,其中Fe代谢相关梭菌属(Clostridium)主导的自养反硝化在共混系统脱氮过程中可能发挥着重要作用.
In response to the requirements for advanced nitrate removal in wastewater treatment plants,pyrite(FeS_(2))and poly-3-hydroxybutyrate-co-3-hydroxyvalerate(PHBV)were selected to set up a combined autotrophic-heterotrophic denitrification packed bed bioreactor for treating secondary effluent of wastewater treatment plants.Through monitoring influent and effluent water quality,material structure characterization(SEM),and16 S rDNA,the operation effect and mechanism of the combined autotrophic-heterotrophic denitrification system were explored.Results showed that the denitrification rate of FeS_(2)-PHBV synergistic denitrification system is better than that of FeS_(2) autotrophic system alone.When the hydraulic retention time was 2 h,the denitrification efficiency could reach more than 90%,and the sulfate produced was less.Compared with the heterotrophic PHBV system alone,the synergistic system achieved the same total nitrogen removal performance while releasing less DOC(23.62 mg/L).In the FeS_(2)-PHBV system,the surface structures of the two materials changed significantly,which was due to the enrichment and utilization of biofilms on the material surface.The presence of the dominant bacteria,including Sporomusaceae and Clostridium sensu stricto 7 FeS_(2)-PHBV system,realized the synergistic denitrification of heterotrophic and autotrophic.Therein,the autotrophic denitrification driven by Fe-related Clostridium may play an important role in nitrogen removal in the FeS_(2)-PHBV system.
作者
吴为中
赵柳
周琦
贾利霞
WU Weizhong;ZHAO Liu;ZHOU Qi;JIA Lixia(College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China)
出处
《应用基础与工程科学学报》
EI
CSCD
北大核心
2022年第2期282-294,共13页
Journal of Basic Science and Engineering
基金
国家自然科学基金面上项目(52070001)。
关键词
黄铁矿
固相碳源
自养-异养反硝化
填充床生物反应器
生物膜
优势菌
pyrite
solid carbon source
autotrophic and heterotrophic denitrification
pack bed bioreactor
biofilm
dominant bacteria
