摘要
目的:利用高效液相色谱构建同时对蔬菜中所残留的氯氰菊酯、联苯菊酯、噻虫嗪以及吡虫啉4种农药进行测定的方法。方法:以乙腈为提取剂,净化后使用体积比为1∶19的甲醇+二氯甲烷混合液进行洗脱,利用高效液相色谱仪进行测定。结果:氯氰菊酯、联苯菊酯、噻虫嗪、吡虫啉在0.02~0.30 mg·mL^(-1)的质量浓度区间内呈现出线性相关关系,相关系数为0.9995~0.9999,其对应的检出限为0.05 mg·kg^(-1)、0.05 mg·kg^(-1)、0.05 mg·kg^(-1)、0.05 mg·kg^(-1)。结论:方法相对较为简便快捷,且精准度较高,具有很强的实用性,能够满足日常检测蔬菜中农药残留的需求。
Objective:To establish a method for the simultaneous determination of four pesticides,cypermethrin,bifenthrin,thiamethoxam and imidacloprid in vegetables,by using high performance liquid chromatography.Method:Acetonitrile was used as the extracting solution,and after purification,the mixture of methanol and dichloromethane with a volume ratio of 1:19 was used for elution,and the determination was carried out by high performance liquid chromatography.Result:Cypermethrin,bifenthrin,thiamethoxam and imidacloprid showed a linear correlation in 0.02~0.30 mg·mL^(-1)certain mass concentration range,the correlation coefficient was 0.9995~0.9999,and the corresponding detection limits were 0.05 mg·kg^(-1),0.05 mg·kg^(-1),0.05 mg·kg^(-1),0.05 mg·kg^(-1).Conclusion:The method is relatively simple,fast,and has high accuracy and strong practicability,it can meet the needs of daily detection of pesticide residues in vegetables.
作者
郑碧秋
ZHENG Biqiu(Maoming Testing Institute for Food and Drug Control,Maoming 525000,China)
出处
《现代食品》
2022年第13期161-163,186,共4页
Modern Food
关键词
高效液相色谱法
蔬菜
农药残留
high performance liquid chromatography
vegetables
pesticide residues
