摘要
目的建立一种稳定的大鼠皮肤热损伤模型,并探究热损伤对大鼠皮肤choke血管的影响。方法雄性SD大鼠114只,采用随机数字表法分成3组,应用100℃沸水对位于大鼠背部的三血管体穿支区皮肤按实验分组接受10、15或20 s烫伤,每组38只。烫后应用激光散斑灌注成像技术监测皮肤血液灌注变化,采用明胶-氧化铅灌注观察皮肤血管网的密度变化,通过组织学验证各组的热损伤深度并观察其对皮肤choke血管的影响和血管再生情况。计量资料以x±s表示,采用t检验、单因素方差分析或重复测量的方差分析进行统计学分析。结果组织学染色验证了100℃沸水接触皮肤10、15、20 s分别对大鼠背部皮肤造成了浅Ⅱ度、深Ⅱ度和Ⅲ度的热损伤。激光散斑灌注成像显示,在烫后第7天,热损伤10 s组的血流灌注量恢复至烫前水平[皮肤血流散斑流速指数(SFI):143.25±30.40 vs.140.28±26.35,P=0.828],热损伤15 s组SFI较烫前略有减低,但差异无统计学意义(106.20±10.30 vs.119.31±9.66,P=0.072),而热损伤20 s组SFI较烫前明显减低(67.49±19.93 vs.136.37±18.96,P=0.001)。明胶-氧化铅灌注显示,在烫后第14天,热损伤10 s组血管密度恢复至烫前;热损伤15 s组血管密度略高于烫前水平;热损伤20 s组血管密度明显低于烫前水平。组织学染色显示,在烫后第7天,热损伤10 s组微血管密度[(29.16±2.38)条/mm^(2)vs.(27.74±3.66)条/mm^(2),P=0.696]和管径[(35.61±2.49)μm vs.(41.74±3.31)μm,P=0.938]较烫前差异无统计学意义;热损伤15 s组微血管密度高于烫前[(36.68±4.65)条/mm^(2)vs.(27.74±3.66)条/mm^(2),P=0.027],而管径较烫前差异无统计学意义[(52.88±4.97)μm vs.(41.74±3.31)μm,P=0.058];热损伤20 s组与烫前相比微血管密度差异无统计学意义[(30.80±2.27)条/mm^(2)vs.(27.74±3.66)条/mm^(2),P=0.407],管径小于烫前[(37.57±5.33)μm vs.(41.74±3.31)μm,P=0.001]。结论浅Ⅱ度热损伤对choke区血管无明显影响;深
Objective To establish a standardized burn injury in rat model and to explore effect of the burn injury to choke vessel of dorsal skin flap in the rat model.Methods 114 male Sprague Dawley rats were randomly divided into 3 groups,38 rats of each group.The dorsal three-perforasome perforator flap of rats in different groups was contacted with 100℃water for 10,15 or 20 seconds,respectively.Blood perfusion of the injured area was monitored for 7 days using PeriCam PSI system.Lead oxide-gelatine angiogram was performed to observe distribution of skin vessels.Histological analysis was performed to observe depth of burn injury and quantify the microvascular density and diameter.The measurement datas were expressed as Mean x±SD,and statistical analysis was performed by t-test,one-way ANOVA and resuable ANOVA.Results 10,15,20-s scald resulted in a superficial second-degree burns,a deep second-degree burns,and deep third-degree burns,respectively.At day 7 after burn injury,perfusion was comparable with the pre-burn level in the 10 s-scalding group[speckle flow index(SFI):143.25±30.40 vs.140.28±26.35,P=0.828],was slightly lower than pre-burn level in the 15 s-scalding group(106.20±10.30 vs.119.31±9.66,P=0.072),and significantly decreased in the 20 s-scalding group(67.49±19.93 vs.136.37±18.96,P=0.001).The density of the blood vessels in the entire three-perforasome area recovered to the pre-burn level within 14 days in the 10 s-scalding group,slightly increased in the 15 s-scalding group,and obviously decreased in the 20 s-scalding group.At day 7 after burn injury,the microvascular density in the choke vessel zone was equal to the pre-burn level in the 10 s-scalding group[(29.16±2.38)/mm^(2) vs.(27.74±3.66)/mm^(2),P=0.696]and 20 s-scalding group[(30.80±2.27)/mm^(2) vs.(27.74±3.66)/mm^(2),P=0.407],and significantly increased in the 15 s-scalding group[(36.68±4.65)/mm^(2) vs.(27.74±3.66)/mm^(2),P=0.027].At day 7 after burn injury,the microvascular diameter of choke vessels was equal to the pre-burn level in the
作者
陈雪莹
滕利
臧梦青
Chen Xueying;Teng Li;Zang Mengqing(Scar Comprehensive Treatment Center,Plastic Surgery Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100144,China)
出处
《中华整形外科杂志》
CSCD
2022年第6期680-690,共11页
Chinese Journal of Plastic Surgery
基金
中国医学科学院整形外科医院科学基金(YS202017)。
