摘要
目的了解食源性金黄色葡萄球菌对常用抗生素的耐药趋势,肠毒素基因分布情况。方法选取食源样本分离并保存的56株金黄色葡萄球菌菌株,采用琼脂稀释法检测对常用抗生素耐药情况。采用PCR热扩增肠毒素和耐药基因并进行分析。结果56株金黄色葡萄球菌中分离出9株MRSA。MRSA对红霉素、青霉素G、头孢唑啉和头孢西丁完全耐药,对替考拉宁和万古霉素未产生耐药性,对四环素、利福平、复方新诺明、庆大霉素和左氧氟沙星耐药率为60.00%、20.00%、20.00%、60.00%和40.00%。MSSA对红霉素、青霉素G、头孢唑啉、四环素、复方新诺明、庆大霉素和左氧氟沙星耐药率为45.10%、60.78%、9.80%、35.29%、9.80%、13.73%和9.80%,未对头孢西丁、利福平、替考拉宁和万古霉素产生耐药性。携带SEA-SEJ基因株数分别为:7、3、1、4、2、4、1、2和1株。携带mecA、ermA、ermB、ermC、aph(3’)-Ⅲa和aac(6’)-aph(2″)的金黄色葡萄球菌分别为:5、8、1、11、3和6株。结论食源性金黄色葡萄球菌普遍存在耐药性,携带SEA型肠毒素金黄色葡萄球菌是主要流行株。
Objective To understand the drug resistance trend of foodborne Staphylococcus aureus to common antibiotics and the distribution of enterotoxin genes.Methods 56 strains of S.aureus isolated and preserved from food samples were selected,and the resistance to common antibiotics was detected by agar dilution method.Enterotoxin and drug resistance genes were amplified and analyzed by PCR.Results 9 strains of MRSA were isolated from 56 strains of S.aureus.MRSA was completely resistant to erythromycin,penicillin G,cefazolin and cefoxitin,but not to teicoplanin and vancomycin.The resistance rates to tetracycline,rifampicin,cotrimoxazole,gentamicin and levofloxacin were 60.00%,20.00%,20.00%,60.00%and 40.00%.The resistance rates of MSSA to erythromycin,penicillin G,cefazolin,tetracycline,cotrimoxazole,gentamicin and levofloxacin were 45.10%,60.78%,9.80%,35.29%,9.80%,13.73%and 9.80%.There was no resistance to cefoxitin,rifampicin,teicoplanin and vancomycin.The number of plants carrying SEA-SEJ gene was 7,3,1,4,2,4,1,2 and 1 respectively.S.aureus carrying mecA,ermA,ermB,ermC,aph(3’)-Ⅲa and aac(6’)-aph(2’’)were 5,8,1,11,3 and 6 strains respectively.Conclusion Drug resistance is common in foodborne S.aureus,and S.aureus carrying SEA enterotoxin is the main epidemic strain.
作者
罗曼
刘倩
LUO Man;LIU Qian(School of Nursing in Chifeng University,Chifeng 024000,Inner Mongolia,China;School of Basic Medical Sciences in Chifeng University)
出处
《中国病原生物学杂志》
CSCD
北大核心
2022年第6期685-688,共4页
Journal of Pathogen Biology
关键词
金黄色葡萄球菌
肠毒素
耐药机制
Staphylococcus aureus
enterotoxin
drug resistance mechanism
