摘要
Bacterial keratitis is the most common corneal infection which may lead to blindness,and seriously threatened the human visual health worldwide.Clinical treatment with antibiotic eye drops formulation usually falls in low bioavailability and poor therapeutic efficiency.Hydrogel has gained much attention as ophthalmic formulation recently due to the prolonged drug retention on ocular surface.In this study,we proposed a type of all-small-molecule supramolecular hydrogel assembled from guanosine-5′-monophosphate disodium salt and tobramycin for the treatment of bacterial keratitis.Guanosine-5′-monophosphate disodium salt assembled into guanosine-quartet nanofibers via hydrogen bonding andπ-πstacking,and tobramycin with five primary amine groups further crosslinked the nanofibers bearing multiple phosphate moieties into gel networks via ionic interactions.The supramolecular gel showed shear thinning and thixotropic properties,good biocompatibility,and antibacterial activity.The gel treatment significantly ameliorated P.aeruginosa induced bacterial keratitis,and showed higher therapeutic efficacy compared to tobramycin eye drop.This study provides a facile and efficient antibiotic gel formulation for clinical treatment of bacterial keratitis.
基金
from the Natural Science Foundation of Shanghai(19ZR1415600)
the National Natural Science Foundation of China(81970766 and 82171102)
the Program for Professor of Special Appointment(Estern Scholar)at Shanghai Institutions of Higher Learning,the Shanghai Innovation Development Program(2020-RGZN-02033)
the Shanghai Key Clinical Research Program(SHDC2020CR3052B)
the Fundamental Research Funds for the Central Universities.
