摘要
目的使用高效液相色谱法(HPLC)和解吸电喷雾电离-离子化质谱成像(DESI-MSI)法研究不同生长年限黄连次生代谢产物的动态积累及分布规律。方法以一、三、五年生黄连为研究对象,采用HPLC法对黄连次生代谢产物的7种指标性成分(盐酸药根碱、非洲防己碱、表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱、木兰花碱)进行含量测定。同时,利用DESI-MSI法对一、三、五年生黄连切片的7种成分的分布进行定性分析。结果HPLC法测定结果显示,盐酸小檗碱、盐酸巴马汀、盐酸药根碱、非洲防己碱、木兰花碱在五年生黄连中含量最高;盐酸黄连碱与表小檗碱在三年生黄连中含量最高,而木兰花碱与盐酸药根碱在三年生黄连中含量最低;盐酸小檗碱、盐酸巴马汀、非洲防己碱、表小檗碱及盐酸黄连碱均在一年生黄连中含量最低。DESI-MSI法测定结果显示,生物碱的盐酸盐在质谱分析过程中解离,最终以原型形式呈现。小檗碱和表小檗碱集中分布于整个黄连切片断面,且一年生分布区域最小、三年生和五年生分布区域相近;药根碱和非洲防己碱分布于皮部和髓部,且以五年生分布区域最大;巴马汀和黄连碱分布于皮部和髓部,一年生分布区域最小;木兰花碱仅分布于木部,且在一、三、五年生黄连中分布区域相近。结论一年生黄连中各成分累积较少,第一年为黄连生长发育的主要阶段;三年生黄连中各成分累积较多,1~3年是黄连次生代谢产物累积的主要时期;五年生黄连中总成分含量最高,尤其是小檗碱累积最多,与以栽培5年以上的黄连为药材的市场习惯相符合。
Objective To use the high-performance liquid chromatography(HPLC)method and desorption electrospray ionization-mass spectrometry imaging(DESI-MSI)method to study the dynamic accumulation and distribution of secondary metabolites of Coptis chinensis in different growth years.Methods Using the samples of one-,three-,and five-year Coptis chinensis as the research object,the HPLC method was used to determine the contents of seven index components(jatrorrhizine hydrochloride,columbamine,epiberberine,coptisine hydrochloride,palmatine hydrochloride,berberine hydrochloride,and magnoflorine)as the secondary metabolites of Coptis chinensis.Further,the distribution of seven index components in the one-,three-and five-year Coptis chinensis slices were qualitatively analyzed by using the DESI-MSI method.Results The HPLC result showed that the contents of berberine hydrochloride and palmatine hydrochloride,jatrorrhizine hydrochloride,columbamine,and magnoflorine in five-year Coptis chinensis were the highest;coptisine hydrochloride and epiberberine reached the highest level in 3-year Coptis chinensis,while the contents of magnoflorine and jatrorrhizine hydrochloride were the lowest in three-year Coptis chinensis;and berberine hydrochloride,palmatine hydrochloride,columbamine,epiberberine,and coptisine hydrochloride were the lowest in one-year Coptis chinensis.The hydrochloride of each alkaloid was dissociated in the process of mass spectrometry and appeared in the prototype form.The result obtained by the DESI-MSI method show that berberine and epiberberine were concentrated in the whole-slices section,the distribution area of the one-year sample was the smallest,and the distribution areas of the three-and five-year samples were similar.Jatrorrhizine and columbamine were distributed in the phloem and pith,and the five-year sample distribution area was the largest;palmatine and coptisine were distributed in the phloem and pith,and the one-year sample distribution area was the smallest.Magnoflorine was only distributed in the wo
作者
王丹
戚苗
赵禾笛
陈晗
王艳敏
江垭霓
蓝亚楠
王晶娟
WANG Dan;QI Miao;ZHAO Hedi;CHEN Han;WANG Yanmin;JIANG Yani;LAN Yanan;WANG Jingjuan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2022年第6期612-620,共9页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.81872995)。
关键词
黄连
生长年限
次生代谢产物
生物碱
解吸电喷雾电离-离子化质谱成像
高效液相色谱
Coptis chinensis
growth years
secondary metabolites
alkaloids
desorption electrospray ionization-mass spectrometry imaging
high-performance liquid chromatography
