摘要
目的设计两种不同获取微细脂肪颗粒的方法,探讨不同方法获得微细脂肪颗粒的可行性。方法2015年12月至2016年6月,上海交通大学医学院附属第九人民医院对5例女性患者(年龄22~31岁,平均26.2岁)行腹部脂肪抽吸术。分别通过漂浮法和过滤法获取微细脂肪颗粒,与乳糜脂肪和脂肪颗粒进行比较。通过细胞活性、流式分析、组织染色等方式比较各组脂肪组织的细胞活性和组分等指标。结果漂浮法和过滤法获取的微细脂肪颗粒与乳糜脂肪均可通过OT针头。活细胞染色发现两种方法获取脂肪中均存在活性细胞,而乳糜脂肪中未见。流式细胞分析中,CD90漂浮组表达高于对照组(P=0.048)。结论经漂浮法或过滤法处理可获得微细脂肪颗粒,既保留组织完整性和细胞活性,又可实现细针注射与临床应用。
Objective In the process of fat grafting,there is no good solution to achieve delicate filling while retaining the viability of fat particle.There was still no research on the composition and activity of delicate fat particle.In this study,two different methods for delicate fat process were established to explore the feasibility and clinical efficacy.Methods From December 2015 to June 2016,5 patients(22-31 years old,with average 26.2 years)with abdominal liposuction in Shanghai Ninth People's Hospital were inclueded.The fat particles were obtained by floating method and filtration method respectively,and compared with emulsified fat/nanofat and traditional fat grafts.The cell viability and composition of adipose tissue were compared in each group through cell viability test,flow analysis and tissue staining.Results The fat particles obtained by floating and filtration methods,emulsified fat could pass through OT needle.Cell viability test revealed the presence of living cells in the fat obtained by both methods,but not in the emulsified fat.Flow analysis showed that the expression of CD90 in floating group was higher than that in control group(P=0.048).Conclusions The delicate fat particle can be obtained by floating or filtration method,which can preserves the tissue integrity and cell viability;meanwhile it can achieve fine needle injection and clinical application.
作者
程辰
何际洲
李青峰
谢芸
Cheng Chen;He Jizhou;Li Qingfeng;Xie Yun(Department of Plastic and Reconstructive Surgery,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)
出处
《中华医学美学美容杂志》
2022年第3期177-181,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
交通大学医学院双百人计划(20191916)
九院临床研究助力计划资助(JYLJ202103)
上海市临床重点专科建设项目-整形外科(shslczdzk00901)。
