摘要
目的分析程序性坏死特异性抑制剂(Nec-1)鞘内注射抑制炎性反应对大鼠外周神经病理性疼痛的影响。方法选取45只雄性SD大鼠为研究对象,将45只大鼠按照随机数字表法分为对照组、损伤组、损伤给药组,每组15只。对照组为空白组不做任何处理,损伤组给予坐骨神经损伤,损伤给药组给予坐骨神经损伤,并在术后5 d给予Nec-1鞘内注射治疗,分别于术前1 d,术后1、3、5、7、10、12、14 d观察3组大鼠热刺激缩足反射潜伏期(TWL)、机械缩足反射阈值(MWT)。术后14 d采用酶联免疫吸附试验检测所有大鼠肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6水平,将所有大鼠麻醉处死,取L_(4)~L_(6)段脊髓背角,使用Western blot法检测大鼠混合系激酶区域样蛋白(MLKL)、受体相互作用蛋白(RIP)1、RIP3水平。结果3组大鼠术前1 d TWL、MWT比较,差异无统计学意义(P>0.05)。对照组术前1 d与术后1、3、5、7、10、12、14 d TWL、MWT比较,差异无统计学意义(P>0.05)。损伤组TWL、MWT术后1、3、5、7、10、12、14 d明显下降,与术前1 d比较,差异有统计学意义(P<0.001)。损伤给药组术后1、3、5 d TWL、MWT较术前1 d明显下降,给药后出现升高,术后7、10、12、14 d TWL、MWT较术后1、3、5 d明显升高,差异有统计学意义(P<0.001)。MLKL、RIP1、RIP3水平在对照组、损伤给药组、损伤组依次升高,两两比较,差异有统计学意义(P<0.001)。TNF-α、IL-1β、IL-6水平在对照组、损伤给药组、损伤组中依次升高,两两比较,差异有统计学意义(P<0.001)。结论Nec-1鞘内注射可以对大鼠外周神经病理性疼痛产生积极影响,还可有效抑制脊髓背角细胞的坏死性凋亡,降低大鼠炎症因子水平。
Objective To analyze the effect of intrathecal injection of a specific inhibitor of programmed Nec-1 on the peripheral neuropathic pain in rats.Methods A total of 45 male SD rats were selected as the research subjects,and divided into control group,injury group and injury administration group according to random number table method,15 rats in each group.The control group was the blank group without any treatment,injury group was given the sciatic nerve injury,injury administration group was given the sciatic nerve damage,and given Nec-1 intrathecal injection treatment after surgery 5 d,thermal stimulus retraction latency(TWL),mechanical withdrawal reflex threshold(MWT)were observed at 1 d before surgery,1,3,5,7,10,12,14 d after surgery.After surgery 14 d,the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)were determined by enzyme-linked immunosorbent assay,all rats were anesthetized and sacrificed,and the levels of rat hybrid kinase domain-like protein(MLKL),receptor interacting protein(RIP)1 and RIP3 were detected by Western blot.Results There was no significant difference on TWL and MWT among 3 groups 1 day before surgery(P>0.05).There was no significant difference on TWL and MWT between the control group at 1 d before surgery and 1,3,5,7,10,12,14 d after surgery(P>0.05).In the injury group,TWL and MWT decreased significantly at 1,3,5,7,10,12,14 d after surgery,the differences were statistically significant compared with at 1 d before surgery(P<0.001).In the injury administration group,TWL and MWT at 1,3 d and 5 d after surgery decreased significantly compared with at 1 d before surgery,and increased after administration.TWL and MWT at 7,10,12 and 14 d after surgery increased significantly compared with at 1,3 and 5 d after surgery,with statistical significance(P<0.001).The levels of MLKL,RIP1 and RIP3 in control group,injury administration group and injury group increased successively,the differences were statistically significant(P<0.001).The levels of TNF-α,IL-1βand I
作者
张秀梅
ZHANG Xiumei(Department of Anesthesia,Ganzhou People′s Hospital,Ganzhou,Jiangxi 341000,China)
出处
《检验医学与临床》
CAS
2022年第14期1904-1906,1911,共4页
Laboratory Medicine and Clinic
基金
江西省教育厅科学技术研究项目(160996)。
