摘要
为准确鉴定和筛选产纤维素和脂肪酶细菌,通过平板扩散法测定不同氮源培养基对细菌产纤维素酶和不同碳源培养基对细菌产脂肪酶活性的影响,确定细菌产纤维素酶和脂肪酶的最佳培养基,利用最佳培养基研究不同琼脂含量、海水和淡水溶剂、菌种的培养时间及接种后的培养时间对细菌纤维素酶和脂肪酶活性的影响。结果表明,以蛋白胨为氮源的A培养基为细菌产纤维素酶最佳培养基,以Tween-20为碳源的培养基为细菌产脂肪酶最佳培养基;培养基中琼脂的最佳用量均为13‰;所有菌株在海水培养基上产生的纤维素酶活性比淡水培养基上高,但脂肪酶活性并非如此;鉴定和筛选产纤维素酶和脂肪酶细菌接种菌种的最佳培养时间分别为18 h和24~32 h,测定细菌产纤维素酶和脂肪酶的最佳培养时间分别为48~72 h和120 h。
For accurate identification and screening of cellulase and lipase production bacteria,plate diffusion method was used to study the effects of different nitrogen sources on bacterial production of cellulase and different carbon sources on the production of bacterial lipase,so as to identify the optimum medium for cellulase and lipase production.Then different agar content,sea and fresh water solvent,strain′s culture time and culture time after inoculation were studied by using the optimal medium.The results showed that medium A with peptone as nitrogen source was the optimum medium for cellulase production,while the medium with Tween-20 as carbon source was the optimum medium for lipase production.The optimum agar concentration in the medium was 13‰.The cellulase activity of all strains on seawater medium were higher than that on fresh water medium,but lipase activity was not the same.The optimum culture time of inoculums for cellulase-and-lipase-producing strains characterization and screening was at 24 h and 24-32 h respectively,and the optimum culture time for determination of cellulase-and-lipase-producing strains was 48-72 h and 120 h respectively.
作者
张东升
徐一馨
宋双民
周玮
ZHANG Dong-sheng;XU Yi-xin;SONG Shuang-min;ZHOU Wei(Coll.of Fish.&Life Sci.,Dalian Ocean Uni.,Dalian 116023;Key Lab.of Mar.Aquacult.in N.China,Minist.of Agric.,Dalian Ocean Uni.,Dalian 116023)
出处
《微生物学杂志》
CAS
CSCD
2022年第3期44-53,共10页
Journal of Microbiology
基金
辽宁省科学计划项目(2018104009)
辽宁省“兴辽人才”项目(XLYC1808029)
大连市第二届领军人才资助项目
大连市重点领域创新团队支持计划项目(2019RT11)。
