摘要
为了满足粮食谷物中伏马毒素B1快速定量检测的需求,以伏马毒素B1为研究对象,建立了基于时间分辨荧光纳米微球的FB1荧光快速定量检测卡,检测前无需调整样品提取液pH,并借助酶联免疫试剂盒及高效液相色谱法分析比对了该荧光定量快速检测卡在粮食谷物(大米、玉米、小麦)中的检测性能。结果表明其检测不同样品的LOD在35.37~37.17μg/kg之间,LOQ在117.9~123.9μg/kg之间,灵敏度良好;线性范围均为250~6000μg/kg,相关系数R2在0.9972~0.9995之间;不同样品中6个添加水平的加标回收率为83.38%~114.66%,变异系数(CV)小于15%,准确性和重复性良好;国标液相色谱法和伏马毒素B1荧光定量快速检测卡同时检测FB1污染的不同样品,检测结果的符合度为92.53%~106.26%,CV小于10.37%;与其他常见的5种真菌毒素的交叉反应率均小于5%,且添加浓度和检出浓度的差异极显著,表明特异性良好。因此,所制备的FB1荧光定量快速检测卡能够满足粮食谷物中伏马毒素B1现场化快速定量检测的需求。
In order to meet the needs of rapid quantitative detection of fumonisin B1(FB1)in grains,this paper takes fumonisin B1 as the research object,and creates a rapid quantitative detection card of FB1 on the basis of time-resolved fluorescent nanospheres,with no need to adjust the PH of samples before detection.The detection performance of the detection card in grains(rice,corn,wheat)was analyzed and compared with enzyme-linked immunosorbent assay kit and high performance liquid chromatography.The LOD for different samples was in the range of 35.37~37.17μg/kg,and the LOQ was 117.9~123.9μg/kg,which meant good sensitivity;the linear range was 250~6000μg/kg,while the correlation coefficient R2 was in the range of 0.9972~0.9995;the spiking recoveries at 6 spiked levels in different samples were in the range of 83.38%~114.66%,with the coefficient of variation(CV)less than 15%,which meant good accuracy and repeatability.When the national standard liquid chromatography and FB1 fluorescence quantitative rapid detection card simultaneously detects different samples contaminated by FB1,the coincidence of the detection results was in the ranger of 92.53%~106.26%,with the CV less than 10.37%.The cross-reaction rate of other five common mycotoxins was less than 5%.Meanwhile,the difference between the spiked concentration and their average detected concentration was extremely significant,indicating good specificity.Hence,the FB1 fluorescence quantitative detection card prepared in this study could meet the needs of on-site rapid quantitative detection of fumonisin B1 in grains.
作者
刘洪美
郑梦瑶
袁华山
王平东
赵皖
肖理文
叶金
王松雪
LIU Hong-mei;ZHENG Meng-yao;YUAN Hua-shan;WANG Ping-dong;ZHAO Wan;XIAO Li-wen;YE Jin;WANG Song-xue(Institute of Grain and Oil Quality and Safety,Academy of National Food and Strategic Reserves Administration,Beijing 102629,China;Nanjing Storage Grain Oil Quality Supervision Center Co.,Ltd.,Nanjing,Jiangsu 211151,China;Nanjing Microtest Biotechnology Co.,Ltd.,Nanjing,Jiangsu 210031,China)
出处
《粮油食品科技》
2022年第4期164-171,共8页
Science and Technology of Cereals,Oils and Foods
基金
中国科协青年人才托举工程项目(2021QNRC001)
国家自然科学基金(81803712)
中央级公益性科研院所基本科研业务费专项(ZX1922,ZX2001)
广西重点研发计划(桂科AB20238018)。
关键词
伏马毒素B1
快速定量检测
时间分辨荧光
纳米微球
荧光定量免疫层析
Fumonisin B1
rapid quantitative detection
time-resolved fluorescent
nanospheres
fluorescence quantitative immunochromatography
