摘要
【目的】皂刺中的许多次生代谢物是药用活性成分。本文旨在探究皂荚与野皂荚刺的差异次生代谢物及进行差异代谢途径富集分析,为皂刺功能性化合物分离鉴定以及研究开发提供理论依据。【方法】以皂荚和野皂荚的1年生棘刺为材料,用UPLC-MS/MS检测平台和自建数据库MWDB4.0进行代谢组分析。【结果】1)2个种的皂刺中共检测到457种次生代谢物,分为酚酸、生物碱、萜类、黄酮、木脂素、香豆素、鞣质类、其他8大类,酚酸和黄酮占比较大;2)野皂荚刺与皂荚刺相比,共有213种显著差异代谢物,其中121种物质在野皂荚刺中含量较高,包括55种酚酸,54种黄酮,5种木脂素和香豆素,4种生物碱,2种萜类,1种其他;92种物质在皂荚刺中含量较高,包括21种酚酸,19种黄酮,14种鞣质,14种生物碱,8种木脂素和香豆素,16种其他。3)皂荚刺中特有的代谢物有22种,异牡荆素-2″-O-鼠李糖苷、N,N′-二阿魏酰腐胺、香叶木素-8-C-(2″-O-鼠李糖基)葡萄糖苷、紫檀芪、原花青素C2相对含量较高,野皂荚刺中特有的代谢物有27种,松柏苷、5-O-咖啡酰莽草酸、槲皮素-3-O-(2″-对香豆酰)葡萄糖苷的相对含量较高。4)差异代谢物的代谢途径分析表明“茋类、二芳基庚烷类和姜酚的生物合成”途径中显著差异代谢物富集显著,苯丙烷生物合成和类黄酮代谢的差异代谢物富集较多。【结论】皂荚和野皂荚刺中共检测到457种次生代谢物;差异代谢物质主要是黄酮和酚酸类;茋类、二芳基庚烷类和姜酚的生物合成的差异代谢物显著富集(P<0.05),苯丙烷生物合成和黄酮类生物合成的差异代谢物富集较多。
【Objective】Many secondary metabolites in spina gleditsiae are medicinal active ingredients.This article aims to investigate the differential secondary metabolites in spines of Gleditsia sinensis and Gleditsia microphylla,and analyze the enrichment of the differential metabolic pathway,so as to provide a theoretical basis for the separation and identification of functional compounds from spina gleditsiae and for the research and development of functional compounds.【Method】The 1-year-old spines of G.sinensis and G.microphylla were used as materials,the UPLC-MS/MS detection platform was used to determine secondary metabolites,and self-built database MWDB4.0 was used for metabonomic analysis.【Results】1)A total of 457 secondary metabolites were detected in the two species of spina gleditsiae,which were divided into 8 categories:phenolic acids,alkaloids,terpenes,flavonoids,lignans,coumarins,tannins,and others.Among them,phenolic acids and flavonoids accounted for a relatively large proportion;2)There were significant differences in 213 metabolites in the spines between G.sinensis and G.Microphylla,of which the contents of 121 substances were higher in G.microphylla spines,including 55 phenolic acids and 54 flavonoids,5 kinds of lignans and coumarins,4 alkaloids,2 terpenes,and 1 other substance;The contents of 92 substances in G.microphylla were lower,including 21 phenolic acids,19 flavonoids,14 tannins,14 alkaloids,8 kinds of lignans and coumarins,16 others.3)There were 22 kinds of unique metabolites in G.sinensis spines,of which isovitexin-2″-O-rhamnoside,N,N′-Diferuloylputrescine,diosmetin-8-C-(2″-O-rhamnosyl)glucoside,pterostilbene,and procyanidin C2 had relatively higher content.There were 27 unique metabolites in G.microphylla spines,of which the relative content of coniferin,5-O-Caffeoylshikimic acid,and quercetin-3-O-(2″-p-Coumaroyl)glucoside was relatively higher.4)Metabolic pathway analysis of differential metabolites showed that the significantly differential metabolites in the pathways of
作者
耿瑜欣
李红姣
郑建伟
张芹
于丽娜
李佳秋
李保会
Geng Yuxin;Li Hongjiao;Zheng Jianwei;Zhang Qin;Yu Lina;Li Jiaqiu;Li Baohui(School of Forestry,Agricultural University of,Hebei Baoding 071000;School of Landscape Architecture and Tourism,Agricultural University of Hebei Baoding 071000)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2022年第4期82-94,共13页
Scientia Silvae Sinicae
基金
河北省重点研发计划项目(19226808D)。
关键词
皂荚
野皂荚
皂刺
代谢组
差异代谢物
通路富集分析
Gleditsia sinensis
Gleditsia microphylla
spina gleditsiae
metabolome
differential metabolites
pathway enrichment analysis
