摘要
扭果花旗杆(Dontostemon elegans Maxim)是新疆达坂城荒漠地区广泛分布的旱生植物,在维持荒漠植被的稳定性中发挥重大作用。为了揭示干旱胁迫下扭果花旗杆整个基因组水平的表达特性,对25%PEG胁迫处理12 h、72 h的扭果花旗杆幼苗进行了转录组测序分析。经拼接组装共获得64672 Unigene,平均长度为753 bp,N50长度为1527 bp。与七大数据库进行注释,共有48046条Unigene获得了基因注释,占总Unigene的74.29%。其中有29633条Unigene获得了GO注释。通过差异表达基因GO分析显示,608个差异基因主要涉及植物型细胞壁组织或生物发生、细胞壁组织结构成分等功能。KEGG pathway富集分析共有16742 Unigene参与了131个代谢通路,差异基因主要富集到了苯丙素生物合成,类胡萝卜素的生物合成,蜡质、角质的生物合成及二苯乙烯、二芳基庚烷和姜酚生物合成等代谢途径。从扭果花旗杆转录组筛选出来的上调基因多为植物细胞壁组成成分中木质素、纤维素、蜡质、角质、脱落酸等物质的生物合成相关基因。研究表明扭果花旗杆能够通过促进生物合成代谢途经和细胞壁构建途径提高干旱胁迫适应能力,本研究结果有助于揭示扭果花旗杆对干旱的应答机理,为下一步筛选和挖掘抗旱功能基因提供数据支持。
Dontostemon elegans Maxim is a widely distributed xerophyte in Dabancheng desert area of Xinjiang,which plays an important role in maintaining the stability of desert vegetation. In order to reveal the whole genome level expression characteristics of Dontostemon elegans under drought stress, transcriptome sequencing analysis was carried out in seedlings treated with 25% PEG for 12 and 72 h. A total of 64 672 Unigene with an average length of 753 bp and an N50 length of 1 527 bp were obtained by splicing and assembling. 48 046 Unigenes were annotated with seven databases, accounting for 74.29% of the total Unigenes. Among them, 29 633 Unigenes obtained GO annotations. Go analysis showed that 608 differentially expressed genes were mainly involved in plant cell wall tissue or biogenesis, cell wall structure components and other functions. A total of 16 742 Unigenes were involved in 131 metabolic pathways by KEGG pathway enrichment analysis. The differential genes were mainly enriched in phenylpropanoid biosynthesis, carotenoid biosynthesis, wax and cutin biosynthesis, and stilbene, diarylheptane and gingerol biosynthesis. Most of the up-regulated genes screened from Dontostemon elegans transcriptome were related to the biosynthesis of lignin, cellulose, wax, cutin and abscisic acid in plant cell wall components. This study is helpful to reveal the mechanism of response to drought and provide data support for screening and mining drought resistant functional genes in the next step.
作者
马薇
邹丽媛
赵惠新
葛风伟
Ma Wei;Zou Liyuan;Zhao Huixin;Ge Fengwei(Key Laboratory of Plant Stress Biology in Arid Land,Key Laboratory of Special Environment Biodiversity Application and Regulation in Xinjiang,Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,College of Life Science,Xinjiang Normal University,Urumqi,830054)
出处
《分子植物育种》
CAS
北大核心
2022年第11期3548-3561,共14页
Molecular Plant Breeding
基金
自治区高校科研计划科学研究重点项目(XJEDU2016I034)
新疆师范大学博士启动基金(XJNUBS1541)
新疆师范大学“十三五”校级重点学科生物学学科共同资助。
关键词
扭果花旗杆
干旱胁迫
转录组
细胞壁合成
Dontostemon elegans Maxim
Drought stress
Transcriptome
Cell wall synthesis
