摘要
【目的】了解泰山白首乌根际土壤微生物群落结构和组成,为充分利用根际微生物资源,优选根际促生菌、提高仿野生种植技术提供理论依据及科学支撑。【方法】采用Illumina MiSeq高通量测序技术,分析测定3个产地2个根茎深度的泰山白首乌根际土壤细菌群落的丰富度和多样性。【结果】测序结果显示,6个泰山白首乌根际土壤样品共得到细菌5 404个OTUs,分属于22门63纲98目170科274属,其中样品LW20的优势菌门为酸杆菌门Acidobacteria,相对丰度为24.76%;样品LW10、LX10、LX20、CQ10、CQ20的优势菌门均为变形菌门Proteobacteria,相对丰度分别为57.89%、46.00%、38.22%、27.29%、31.05%;样品LW10的优势属为假单胞菌属Pseudomonas,相对丰度为22.35%;样品LW20的优势属为Spartobacteria-genera-incertae-sedis,相对丰度为21.37%;样品LX10的优势属为土壤红杆菌属Solirubrobacter,相对丰度为5.57%;样品LX20的优势属为鞘氨醇单胞菌属Sphingomonas,相对丰度为4.31%;样品CQ10的优势属为norank-Acidobacteria-Gp4,相对丰度为9.58%;样品CQ20的优势属为Streptophyta,相对丰度为9.02%。【结论】综合比较,样品CQ10的细菌群落丰度和多样性最高,Chao和Ace指数分别为1 150.41和1 102.51;样品LX10的细菌群落分布最均匀,Shannoneven指数为0.8315;随着地下根茎深度加深,细菌微生物群落的丰度和多样性降低,不同产地和根茎深度样品间土壤细菌群落存在显著差异性。
【Objective】The study was conducted to understand the structure and composition of rhizosphere soil microbial community of Cynanchum bungei Decne,which provided theoretical basis and scientific support for making full use of rhizosphere microbial resources,optimizing rhizosphere growth-promoting bacteria and improving imitation wild planting technology.【Method】With Illumina MiSeq high-throughput sequencing technology,the richness and diversity of rhizosphere soil bacterial communities of Cynanchum bungei Decne were analyzed and determined.【Result】According to the sequencing results,a total of 5 404 OTUs were obtained from 6 rhizosphere soil samples,belonging to 22 phyla,63 classes,98 orders,170 families and 274 genera,among which the dominant phyla of LW20 was Acidobacteria,with a relative abundance of 24.76%;the dominant phyla of LW10,LX10,LX20,CQ10 and CQ20 were Proteobacteria,with a relative abundance of 57.89%,46.00%,38.22%,27.29% and 31.05%,respectively;the dominant genus of LW10 was Pseudomonas,with a relative abundance of 22.35%;the dominant genus of sample LW20 was Spartobacteria general incertae sedis,and the relative abundance was 21.37%;the dominant genus of sample LX10 was Solirubrobacter,and the relative abundance was 5.57%;the dominant genus of LX20 was Sphingomonas,and the relative abundance was 4.31%;the dominant genus of CQ10 was Norank acidobacteria GP4,and the relative abundance was 9.58%;the dominant genus of sample CQ20 was Streptophyta,and the relative abundance was 9.02%【Conclusion】Comprehensive comparison showed that the bacterial community abundance and diversity of sample CQ10 were the highest,and Chao and Ace indexes were 1 150.41 and 1 102.51,respectively.The bacterial community distribution of sample LX10 was the most uniform,and Shannoneven index was 0.8315.With the deepening of underground rhizome depth,the abundance and diversity of bacterial microbial community decreased.There were significant differences in soil bacterial communities among samples from different pr
作者
李洁
王飞
徐凌川
LI Jie;WANG Fei;XU Lingchuan(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Yuhua Jingchen Ecological Agriculture Development Co.LTD,Feixian 273418,China)
出处
《广东农业科学》
CAS
2022年第5期75-84,共10页
Guangdong Agricultural Sciences
基金
山东省科技攻关项目(2010GSF275)
山东省教育厅科研项目(J08LH10)。
关键词
泰山白首乌
根际土壤
细菌
微生物群落
高通量测序技术
Cynanchum bungei Decne
rhizosphere soil
bacteria
microbial community
high-throughput sequencing technology
