摘要
采用超声波协同有机溶剂提取了滨海白首乌中的总黄酮。首先,利用单因素试验研究了乙醇浓度(60%~100%)、液料比(10∶1~50∶1)、超声温度(30~70℃)、超声时间(10~50 min)以及超声功率(240~400 W)等因素对黄酮提取量的影响。在此基础上,采用正交设计优化了白首乌总黄酮的提取工艺。结果表明:乙醇浓度对黄酮提取量的影响最大,其次为液料比、超声时间和超声温度;最佳提取工艺组合为乙醇浓度90%、超声时间30 min、液料比20∶1、超声温度40℃。在该组合条件下,白首乌中黄酮提取量达到3.95 mg/g。
The flavonoids were extracted from Cynanchum auriculatum in Binhai by ultrasonic wave and organic solvent.The effects of ethanol concentration(60%~100%),liquid-to-solid mass ratio(10∶1~50∶1),extraction temperature(30~70℃),extraction time(10~50 min),and sonication power(240~400 W)on flavonoid extraction were studied by the single factor test.The extraction technology was further optimized by orthogonal design.The results showed that the ethanol concentration was the most prominent factor determining the extraction yield,followed by liquid-to-solid mass ratio,extraction time,and extraction temperature.The optimum parameters for flavonoids extraction were as follows:liquid-to-solid mass ratio 20∶1,ethanol concentration 90%,extraction temperature 40℃,and extraction time 30 min.The extraction yield of 3.95 mg/g was obtained under the optimized conditions.
作者
孙倩
冯进
李春阳
Chuon Mony Roth
Sun Sovath
Taing Bunhok
SUN Qian;FENG Jin;LI Chun-yang;Chuon Mony Roth;Sun Sovath;Taing Bunhok(College of Chemical Engineering,Nanjing University of Science and Technology,Nanjing 210094,China;Institute of Farm Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Cambodia Laboratory of Agricultural Products and Foods,Ministry of Agriculture Forestry and Fisheries of Cambodia,Phnom Penh 10103,Kingdom of Cambodia)
出处
《江西农业学报》
CAS
2020年第12期60-64,共5页
Acta Agriculturae Jiangxi
基金
2020年江苏省农业科技自主创新资金项目(CX(20)2006)。
关键词
滨海白首乌
黄酮
超声波提取
正交设计
Cynanchum auriculatum of Binhai
Flavonoids
Ultrasonic-assisted extraction
Orthogonal design
