摘要
目的分析辽宁省辽阳市健康人群肠道病毒携带状况和柯萨奇病毒A21型(Coxsackievirus A21,CVA21)基因特征。方法2020年在辽阳市选择健康人群采集粪便标本,进行肠道病毒分离培养;采用逆转录聚合酶链反应进行分离株鉴定和序列测定,分析核苷酸序列同源性。结果研究对象病毒总分离率为5.1%(11/214),其中脊灰病毒、非脊灰肠道病毒(Non-polio enterovirus,NPEV)、腺病毒分离率分别为0.0%、4.2%(9株)、0.9%(2株)。0-6岁、7-14岁、15-19岁、≥20岁人群NPEV分离率分别为0.0%、6.7%(2株)、18.8%(6株)、3.1%(1株)。9株NPEV均鉴定为CVA21,其VP1、VP4区核苷酸同源性分别为99.1%-100%、100%;与广东省参考株的VP1、VP4区核苷酸同源性分别为97.6%、98.5%。结论研究对象携带的肠道病毒均为CVA21且分布于≥7岁人群,未发现脊灰病毒;本研究为辽宁省维持无脊灰状态提供了病原学依据。
ObjectiveTo analyze the prevalence of enterovirus carriage and describe genetic characteristics of Coxsackievirus A21 in a healthy population of Liaoyang city, Liaoning province.MethodsWe recruited healthy individuals in Liaoyang in 2020 and collected stool specimens for enterovirus isolation and culture. We identified enterovirus types and assessed sequences with reverse transcription polymerase chain reaction to determine nucleotide homology.ResultsThe overall positivity rate for virus isolates was 5. 1%(11/214), with 0. 0%, 4. 2%(9 strains), and 0. 9%(2 strains) being poliovirus, non-polio enterovirus( NPEV), or adenovirus, respectively. Positivity rates of NPEV among 0-6-, 7-14-,15-19-, and ≥ 20-year-olds were 0. 0%, 6. 7%( 2 strains), 18. 8%( 6 strains), and 3. 1%(1 strain). The 9 NPEVs were CVA21. Nucleotide homologies in the VP1 and VP4 regions of the 9 NPEV isolates were 99. 1%-100% and 100%, respectively, and were 97. 6% and 98. 5% compared with a Guangdong reference strain.ConclusionsThe enteroviruses isolated from the subjects were enterovirus CVA21, and were found in ≥7-year-olds;no polioviruses were found. The study provides an etiological basis for maintaining polio-free status in Liaoning.
作者
任丽萍
邵玉平
袁琳
王艳
方兴
陈涛
姚文清
Ren Liping;Shao Yuping;Yuan Lin;Wang Yan;Fang Xing;Chen Tao;Yao Wenqing(Liaoning Provincial Center for Disease Control and Prevention,Shenyang 110005,Liaoning,China;Liaoyang Municipal Center for Disease Control and Prevention,Liaoyang 111000,Liaoning,China)
出处
《中国疫苗和免疫》
CSCD
北大核心
2022年第3期268-272,共5页
Chinese Journal of Vaccines and Immunization
基金
国家科技重大专项(2017ZX10103007-001)。
