摘要
【目的】叶绿素荧光可反映盐胁迫下植物光合机构防御机制的受害程度和抗逆性。通过分析盐胁迫对不同耐盐性粳稻种质叶绿素荧光特性的影响,揭示其诱导动力学特征,初步阐明叶绿素荧光相关基因调控粳稻种质苗期耐盐性的机制,为耐盐水稻品种筛选和培育提供理论依据。【方法】以8份耐盐、8份盐敏感粳稻种质为试验材料,于苗期在水培条件下分别测定两组种质0 mmol·L^(-1)和125 mmol·L^(-1) NaCl胁迫3 d、6 d的叶片叶绿素荧光参数。通过主成分分析筛选关键指标,利用隶属函数和标准差系数赋予权重法综合评价各种质资源耐盐性,获得典型耐(敏)盐种质开展叶绿素荧光相关基因OsHCF222和OsABCI7的表达特异性分析。【结果】与对照(CK,0 mmol·L^(-1) NaCl 3 d、6 d)相比,盐胁迫(125 mmol·L^(-1) NaCl 3 d、6 d)可显著降低粳稻种质的最大荧光产量(F_(m))、原初光能转化效率(F_(v)/F_(m))。与CK相比,耐盐种质的非光化学荧光淬灭系数(NPQ)和可变荧光的非光化学猝灭系数(qN)在盐胁迫3 d时显著降低,初始荧光产量(Fo)在盐胁迫6 d显著升高;盐敏感种质的光化学淬灭系数(qP)和qN在盐胁迫3 d时显著降低,实际光化学量子产量(Y)、NPQ、表观光合电子传递速率(ETR)在盐胁迫3 d、6 d时极显著降低。盐胁迫下F_(m)、F_(v)/F_(m)、Y、NPQ和ETR与耐盐级别(STS)均呈极显著正相关,且在耐、敏盐种质间差异显著。通过主成分分析将8个叶绿素荧光参数转换为2个主成分,累积贡献率88.018%;结合各因子载荷大小筛选出F_(m)、F_(v)/F_(m)、Y、NPQ和ETR 5个关键指标,可将16份粳稻种质明确划分为耐盐组和盐敏感组两类。以两个主成分的隶属函数结合权重处理并累加计算盐胁迫下叶绿素荧光特性综合评价值D(DCF),并依此获得16份粳稻种质排名。采用Kinetic模式测定盐胁迫和正常生长(CK)条件下耐盐种质Cigalon、Bertone和盐敏感种质�
【Objective】Chlorophyll fluorescence parameters can reflect the damage degrees and stress resistance of the plant photosynthetic machinery under salt stress.In this study,the analysis of effects of salt stress on chlorophyll fluorescence characteristics of japonica rice with different salt tolerances was performed to reveal its induced kinetic characteristics and preliminarily elucidate the mechanism of OsHCF222 and OsABCI7 regulating salt tolerance of japonica rice at seedling stage,so as to provide a theoretical foundation for screening and breeding salt-tolerant rice varieties.【Method】Eight salt-tolerant and eight salt-sensitive japonica rice germplasm accessions were used as experimental materials in this study.Leaf chlorophyll fluorescence parameters were measured for these materials after treatment using 0 mmol·L^(-1) or 125 mmol·L^(-1) NaCl for 3 or 6 days.Principal component analysis(PCA)was used to screen the key indexes for salt tolerance,and a comprehensive evaluation of japonica rice germplasm was carried out with membership functions and weighted standard deviation coefficient method.The resulting salt-tolerant and salt-sensitive germplasm were used to analyze the relative expressions of OsHCF222 and OsABCI7,two chlorophyll fluorescence related genes.【Result】Compared with the control(CK,0 mmol·L^(-1) NaCl for 3 days or 6 days),the salt stress treatment(125 mmol·L^(-1) NaCl 3 days or 6 days)significantly reduced the maximal fluorescence(F_(m))and maximum quantum efficiency of PSII(F_(v)/F_(m))of japonica rice germplasm.For salt tolerant germplasm accessions,the non-photochemical quenching coefficient(NPQ)and coefficient of non-photochemical quenching of variable fluorescence(qN)decreased significantly at 3 days after salt stress,while the initial fluorescence yield(F_(o))increased significantly at 6 days after salt stress.The photochemical quenching coefficient(qP)and qN of the salt-sensitive germplasm accessions decreased significantly on the 3rd day after salt stress treatment,while t
作者
朱春艳
宋佳伟
白天亮
王娜
马帅国
普正菲
董艳
吕建东
李杰
田蓉蓉
罗成科
张银霞
马天利
李培富
田蕾
ZHU ChunYan;SONG JiaWei;BAI TianLiang;WANG Na;MA ShuaiGuo;PU ZhengFei;DONG Yan;LÜJianDong;LI Jie;TIAN RongRong;LUO ChengKe;ZHANG YinXia;MA TianLi;LI PeiFu;TIAN Lei(College of Agriculture,Ningxia University/Key Laboratory of Modern Molecular Breeding for Dominant and Characteristic Crops in Ningxia,Yinchuan 750021;AVIC(Ning Xia)Biology Co.,Ltd.,Yinchuan 750002;College of Agriculture,Tarim University,Alar 843300,Xinjiang)
出处
《中国农业科学》
CAS
CSCD
北大核心
2022年第13期2509-2525,共17页
Scientia Agricultura Sinica
基金
宁夏自然科学基金(2020AAC03096)
国家自然科学基金(31760374)
宁夏大学研究生创新项目(GIP2021031)
宁夏农业育种专项课题(2018NYYZ0301,2018NYYZ0302)。
关键词
粳稻
盐胁迫
叶绿素荧光参数
诱导动力学曲线
实时荧光定量PCR
Oryza sativa japonica
salt stress
chlorophyll fluorescence parameters
chlorophyll fluorescence induction curves
quantitative real-time PCR
