摘要
为了研究外源Ca^(2+)对高盐胁迫下香蕉(Musa nana Lour.)叶绿素荧光特性的影响,以中国热带亚热带主栽品种巴西蕉(M.paradisiaca)幼苗为材料,500 mmol·L^(-1) NaCl模拟高盐胁迫,5 mmol·L^(-1) CaCl_(2)模拟外源Ca^(2+),分别处理0 h、4 h、8 h、12 h和24 h,利用叶绿素荧光探测技术对叶片叶绿素荧光特性进行分析。结果表明,高盐胁迫下,巴西蕉幼苗叶片的最大光化学效率(F_(v)/F_(m))、光化学淬灭(qL)、相对电子传递速率(rETR)、快速光曲线初始斜率(α)、潜在最大相对电子传递速率(rETR_(max))、半饱和光强(I_(k))随着处理时间的延长而显著下降且非光化光淬灭(qN)显著升高。添加外源Ca^(2+)能显著提高高盐胁迫下巴西蕉幼苗叶片F_(v)/F_(m)、qL、rETR、α、rETR_(max)、I_(k)和qN,增强巴西蕉幼苗的光合活性、光保护能力、捕光能力和强光耐受能力。以上结果表明,外源Ca^(2+)能有效缓解高盐胁迫对巴西蕉幼苗光合系统产生的不利影响。
In order to study the effect of exogenous Ca^(2+)on the chlorophy Ⅱ fluorescence characteristics of Musa spp.under high salt stress,the seedlings of M.paradisiaca,a main tropical and subtropical banana variety,were treated with 500 mmol/L NaCl to simulate high salt stress and 5 mmol/L CaCl_(2) to simulate exogenous Ca^(2+)for 0,4,8,12 and 24 h,respectively,Chlorophy Ⅱ fluorescence characteristics of leaves were analyzed by chlorophy Ⅱ fluorescence detection technology.The results showed that:under high salt stress,the maximum photochemical efficiency(F_(v)/F_(m)) photochemical quenching(qL),relative electron transfer rate(rETR)and the initial slope of fast light curve of M.paradisiaca seedlings were significantly higher than those of the control(α)、The potential maximum relative electron transfer rate(rETR_(max)) and half saturation light intensity(I_(k)) decreased significantly with the increase of treatment time,and the non photochemical light quenching(qN)increased significantly.Adding ogenous Ca^(2+),it can significantly increase F_(v)/F_(m) qL,rETR,α,rERT_(max),IK and qN.The above results showed that high salt stress reduced the photosynthetic activity,light protection ability,light harvesting ability and strong light tolerance of M.paradisiaca seedlings,and exogenous Ca^(2+)could effectively alleviate the adverse effects of high salt stress on the photosystem of banana seedlings.
作者
杨振
徐亚
汪奇
滕梦鑫
王梦娣
李新国
Yang Zhen;Xu Ya;Wang Qi;Teng Mengxin;Wang Mengdi;Li Xinguo(College of Horticulture,Hainan University.Haikou,570228;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)
出处
《分子植物育种》
CAS
北大核心
2022年第11期3768-3775,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760549)资助。
