摘要
目的对不同厂家布鲁菌病胶体金(colloidal gold)抗体检测试剂盒的有效性进行评价,以确保实验室检测结果的可靠性。方法采集178份内蒙古乌兰察布市布病高危人群血清样本,分别用试管凝集试验(tube agglutination test,SAT)和4个厂家的胶体金试纸条进行抗体检测。以SAT检测结果为标准,通过Kappa一致性检验、χ^(2)检验及诊断效能评价,比对分析4种品牌胶体金的检测效果。结果4个厂家胶体金试剂盒的整体一致性较高;1个厂家试剂盒的检测结果与SAT差异有统计学意义(χ^(2)=73.5,P<0.05)。2个厂家试剂盒的灵敏度及符合率较好,均在90%以上,1个厂家中等,在88%左右,1个厂家较差,在55%左右。结论不同厂家的商品化试剂盒检测效果存在差异,在实际监测工作使用中应对商品化试剂盒进行一致性、差异性检验和诊断效能评价,以选择检测效果最佳的试剂盒,从而确保检测效果的客观、准确。
Objective To evaluate the effectiveness of colloidal gold antibody detection kits for brucellosis from different manufacturers,and to ensure the reliability of the laboratory test results.Methods A total of 178 serum samples from the high-risk population for brucellosis in Wulanchabu City,Inner Mongolia were collected and detected for antibodies using tube agglutination test(SAT)and colloidal gold test strips from 4 manufacturers.The test results of four brands of colloidal gold were compared and analyzed based on the SAT results through Kappa consistency test,χ^(2)test and diagnostic efficiency evaluation.Results The overall consistency of colloidal gold kits from four manufacturers was high.There was a significant difference between the test results of the kits from one manufacturer and the SAT(χ^(2)=73.5,P<0.05).The sensitivity and coincidence rate of kits from two manufacturers were better,both above 90%,one manufacturer was medium,about 88%,and one manufacturer was poor,about 55%.Conclusion There are differences in the detection effects of commercial kits from different manufacturers.In the actual monitoring work,the consistency and difference test and diagnostic efficiency evaluation of commercial kits should be carried out to select the kit with the best detection effect,to ensure the objective and accuracy of the detection effect.
作者
赵敖宁
塔娜
范蒙光
宋利桃
尉瑞平
李晓燕
郭威
张文强
王群颖
ZHAO Ao-ning;Tana;FAN Meng-guang;SONG Li-tao;YU Rui-ping;LI Xiao-yan;GUO Wei;ZHANG Wen-qiang;WANG Qun-ying(Inner Mongolia Medical University,Hohhot 010000,China;不详)
出处
《医学动物防制》
2022年第6期514-516,共3页
Journal of Medical Pest Control
基金
国家科技重大专项—“一带一路”重要传染病流行规律研究(2018ZX10101002-001-009)。
