摘要
目的检测房水中VEGF、TGF-β1、IL-6、IL-17A、MCP-1、ICAM和血清CRP在息肉状脉络膜血管病变(Polypoidal Choroidal Vasculopathy,PCV)中的表达,研究相关因子与PCV引起的黄斑水肿的相关性。方法随机选取40例(40眼)在我院确诊为PCV的患者为实验组,20例(20眼)单纯年龄相关性白内障患者为对照组。术前分别采集两组的房水及静脉血。采用微球法检测房水中VEGF、TGF-β1、IL-6、IL-17A、MCP-1和ICAM的浓度,采用免疫比浊法检测血清CRP的水平。分析细胞因子和CRP在PCV中的表达及与PCV引起黄斑水肿的相关性。实验组玻璃体腔注射雷珠单抗注射液。两组患者治疗前行最佳矫正视力(best corrected visual acuity,BCVA)和黄斑区中心凹厚度(central macular thickness,CMT)检测。结果术前实验组与对照组相比,房水中VEGF、TGF-β1、IL-17A、MCP-1和血清CRP水平显著高于对照组(t=21.72、12.32、10.48、8.39、6.54,P<0.01),ICAM和IL-6与对照组差异无统计学意义(P>0.05),实验组患者VEGF、TGF-β1、IL-17A、MCP-1及CRP水平与CMT呈正相关(r=0.323、0.460、0.435、0.482、0.681,P<0.05),VEGF、TGF-β1、IL-17A、MCP-1与CRP呈正相关(r=0.347、0.594、0.411、0.394,P<0.05),ICAM IL-6与CMT无相关性(r=-0.207、-0.153,P>0.05)。结论VEGF、TGF-β1、IL-17A、MCP-1及CRP水平在PCV患者中显著增高,且与CMT呈正相关,可能参与了PCV的发病。
Objective To detect the expression of VEGF,TGF-β1,IL-6,IL-17A,MCP-1 and ICAM in aqueous humor and the expression of CRP in serum of patients with polypoid choroidal vascular disease(PCV),and to study the relationship between these factors and macular edema caused by PCV.Methods A total of 40 cases(40 eyes)diagnosed with PCV in our hospital were randomly selected as the treatment group.Twehty cases(20 eyes)with simple age-related cataract were selected as the control group.Aqueous humor and venous blood were collected before operation.The levels of VEGF,TGF-β1,IL-6,IL-17A,MCP-1 and ICAM in aqueous humor were detected by the microsphere method.The level of CRP in serum was measured by immunoturbidimetry.The correlation between these factors and macular edema caused by PCV were analyzed.The treatment group was given intravitreal injection of Ranibizumab.The best corrected visual acuity(BCVA)and central macular thickness(CMT)were measured before operation.Results The concentration of VEGF,TGF-β1,IL-17A and MCP-1 in aqueous humor and serum CRP in the experimental group were significantly higher than those in the control group(t=21.72,12.32,10.48,8.39,6.54,P<0.01).There was no significant difference in the levels of IL-6 and ICAM between the experimental group and the control group(P>005).The levels of VEGF,TGF-β1,IL-17A,MCP-1 and CRP were positively correlated with CMT(r=0.323,0.460,0.435,0.482,0.681,P<0.05),The levels of VEGF,TGF-β1,IL-17A,MCP-1 were positively correlated with the level of CRP in serum(r=0.347,0.594,0.411,0.394,P<0.05).The levels of ICAM and IL-6 have no correlation with CMT(r=-0.207,-0.153,P>0.05).Conclutions The levels of VEGF,TGF-β1,IL-17A,MCP-1 and CRP were significantly increased in patients with PCV,and were positively correlated with CMT,which may be involved in the pathogenesis of PCV.
作者
杜月
杜红艳
DU Yue;DU Hongyan(Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014060 China;Department of Ophthalmology,Inner Mongolia People's Hospital,Hohhot 010017 China)
出处
《内蒙古医学杂志》
2022年第5期562-565,共4页
Inner Mongolia Medical Journal
