摘要
采用超声辅助溶剂法提取百香果皮中的总黄酮。基于单因素实验结果,以浸膏得率、总黄酮含量和清除DPPH自由基能力多指标为响应值,应用响应面法综合优化总黄酮提取工艺。结果表明,总黄酮最佳工艺为液料比40∶1,乙醇百分含量67%,超声时间30 min。此条件下浸膏得率为12.58%,总黄酮含量为11.56 mg RT/g DW,清除DPPH自由基SC_(50)值为0.19 mg/mL。最优提取工艺下的百香果皮黄酮提取物具有较好的清除ABTS自由基((0.17±0.01)mmol/L TE/g)和铁离子还原能力((2.35±0.03)mmol/L Fe^(2+)/g)。
Total flavonoids are extracted from Passiflora edulis Sims peel by ultrasonic-assisted solvent extraction.Based on the results of single factor experiment,the extraction technology of total flavonoids is optimized by response surface methodology(RSM)with multiple indexes such as extract yield,content of total flavonoids and DPPH free radical scavenging ability as the response values.The results show that the optimal technology is liquid-solid ratio 40∶1,ethanol content 67%,ultrasonic time 30 min.Under these conditions,the extract yield is 12.58%,the content of total flavonoids is 11.56 mg RT/g DW,and the SC_(50) value of DPPH free radical scavenging is 0.19 mg/mL.Under the optimal extraction technology,the flavonoid extract from P.edulis Sims peel has better ABTS radical scavenging ability of((0.17±0.01)mmol/L TE/g)and iron ion reducing ability of((2.35±0.03)mmol/L Fe^(2+)/g).
作者
景继月
许梦圆
田永涛
田海龙
王文蜀
JING Ji-yue;XU Meng-yuan;TIAN Yong-tao;TIAN Hai-long;WANG Wen-shu(Key Laboratory of Ecological Environment in Minority Areas(Minzu University of China),National Ethnic Affairs Commission,Beijing 100081,China;College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China)
出处
《中国调味品》
CAS
北大核心
2022年第7期43-48,共6页
China Condiment
基金
国家民委创新项目团队(10301-202000040203)
建设世界一流大学(学科)和特色发展引导专项(YLDXXK201819)
中央民族大学生命与环境科学学院生物成像与系统生物学研究中心(104-01900403)
中央民族大学创新项目(URTP2021110046,URTP2021110082,URTP2021110062)。
关键词
百香果皮
总黄酮提取
响应面优化
抗氧化活性
Passif lora edulis Sims peel
extraction of total flavonoids
response surface optimization
antioxidant activitiy
