摘要
目的:建立三棱化学成分的超高效液相色谱-紫外法(UPLC-UV)特征图谱,并对主要特征峰进行指认。方法:色谱柱为Waters ACQUITY UPLC HSS T3柱(100 mm×2.1 mm,1.8μm),流动相为乙腈-0.1%磷酸水溶液,线性梯度洗脱,双波长切换检测,0~12 min为300 nm,12~16 min为240 nm,测定10批三棱药材UPLCUV图,确定共有特征峰并计算相似度,利用对照品及超高效液相色谱-四极杆飞行时间质谱法(UPLC-Q/TOF-MS)对主要特征峰进行指认。结果:采集了10批三棱药材UPLC-UV指纹图谱,确定11个特征峰,相似度均大于0.9,认定了9个特征峰,其中通过对照品指认了咖啡酸、对羟基苯甲酸、对香豆酸,通过质谱解析指认了2个苯丙素乙酰蔗糖酯、3个苯丙素甘油酯及首次在三棱中发现的三棱酮。结论:建立了三棱UPLC-UV特征图谱,能够表征不同结构类型的三棱化学成分,为三棱药材质量控制提供了参考。
Objective:To establish the UPLC-UV characteristic fingerprint for Sparganii Rhizoma and identify the main peaks.Methods:The UPLC conditions are as follows:Waters ACQUITY UPLC HSS T3 column(100 mm × 2.1 mm,1.8 μm),mobile phase of acetonitrile-0.1% phosphoric acid aqueous solution,linear gradient elution,and detection at 300nm in 0-12 min and 240 nm in12-16 min.The chromatograms of 10 batches of Sparganii Rhizoma were determined to screen the common peaks and calculate their similarities.The characteristic peaks were characterized by UPLC-Q/TOF-MS and the reference standards.Results:The UPLC-UV chromatograms of10 batches of Sparganii Rhizoma showed 11 common peaks,with similarities>0.9.A total of 9 compounds were identified,with caffeic acid,p-hydroxybenzoic acid,and p-coumaric acid by comparison with the reference standards,and 2 phenylpropanoid acetyl sucrose esters,3 phenylpropanoid glycerols,and sanalengone(discovered for the first time in Sparganii Rhizoma) by MS.Conclusion:The UPLC-UV characteristic fingerprint of Sparganii Rhizoma was established,which can characterize more structure types of chemical components in Sparganii Rhizoma,and thus it is applicable to the quality control of Sparganii Rhizoma.
作者
唐嵩媛
赵琦琦
孙晖
闫广利
王喜军
车延柠
张秀玲
TANG Song-yuan;ZHAO Qi-qi;SUN Hui;YAN Guang-li;WANG Xi-jun;CHE Yan-ning;ZHANG Xiu-ling(Heilongjiang University of Chinese Medicine,Engineering Research Center of Efficiency Evaluation and Industrialization Development of Classical Prescriptions,Ministry of Education,Research Center of Chinmedomics,National Administration of Traditional Chinese Medicine,Harbin 150040,China;Harbin No.4 Chinese Medicine Factory Co.,Ltd.,Harbin 150500,China)
出处
《中国现代中药》
CAS
2022年第6期1009-1017,共9页
Modern Chinese Medicine
基金
黑龙江省应用技术研究与开发计划重大项目(GA18C004)
黑龙江中医药大学科研基金项目(2018xy01)。
