摘要
于2020年从惠州、肇庆、郑州、天津4个城市的超市采集零售鸡肉样品96份,进行粪肠球菌的分离鉴定;采用琼脂二倍稀释法测定肠球菌的最小抑菌浓度(MIC);PCR方法检测肠球菌的耐药基因和毒力基因。结果显示,共分离到41株粪肠球菌(郑州10株,肇庆14株,惠州10株,天津7株),没有分离到屎肠球菌;所有菌株对氨苄西林、万古霉素、利奈唑胺敏感,对四环素、多西环素的耐药率均达到75%以上,对利福平、氯霉素、氟苯尼考、HLSR的耐药率为20%~40%。天津分离株的耐药率最低,惠州地区分离株耐药率最高。在郑州的分离株中检测到1株替加环素耐药菌株。惠州的分离株所携带的耐药基因种类、检出率大多高于其他地区。耐药基因tetL最为流行,检出率高于50%。其次是ermB,检出率为48.78%。17株粪肠球菌检测出optrA基因,检出率为41.46%。已检测的毒力基因中efaA的携带率最高,为95.12%(39/41)。其他依次为gelE 73.17%(30/41)、agg 58.54%(24/41)、asal 36.59%(15/41)、ace 34.15%(14/41)、cylA 9.76%(4/41)。携带的毒力基因数与抗菌药物耐药性之间不存在统计学差异。结果表明,超市零售鸡肉粪肠球菌耐药严重,耐药基因和毒力基因流行且多样,且检测出人医临床重要抗生素耐药基因,应加强对食品动物肉源粪肠球菌耐药性监测。
The antimicrobial resistance, virulence genes of Enterococcus faecalis(E.faecalis) isolates from retail chicken in four supermarkets in different places were investigated and analyzed, aiming to provide theoretical basis for the control of resistance spread of food origin E.faecalis and public health.A total of 96 chicken samples were collected from four different supermarkets in 2020 for isolation and identification of E.faecalis.All the isolates were characterized by antimicrobial susceptibility using agar dilution method and the antimicrobial resistance genes and virulence genes were detected by PCR.The results showed that a total of 41 strains of E.faecalis were obtained.All the strains were sensitive to ampicillin, vancomycin, linezolid.The resistance rate of the strains to tetracycline and doxycycline were above 75%,and the resistance rate of the strains to rifampicin, chloramphenicol, flunfenicol and HLSR were between 20% and 40%.The resistance rate of the strains from Tianjin was the lowest, and that from Huizhou was the highest.One E.faecalis strain from Zhengzhou was resistant to tigecycline.The types and detection rate of resistant genes in Huizhou was higher than that in other places.The drug-resistant gene tetL was the most popular,with a detection rate higher than 50%,followed by ermB with a detection rate of 48.78%.The optrA gene was detected in 17 strains,and the detection rate was 41.46%.Among virulence genes tested,efaA was the most prevalent,with detection rate of 95.12%(39/41),followed by gelE(73.17%,30/41),agg(58.54%,24/41),asal(36.59%,15/41)and ace(34.15%,14/41),agg(9.76%,4/41).There is no statistically difference between the number of virulence genes and drug resistance genes.In conclusion,E.faecalis from retail chicken in supermarket presented serious antimicrobial resistance,antimicrobial-resistant genes and virulence genes are prevalent and diverse among E.faecalis.Genes conferring resistance to relevant antimicrobials in human medicine have also been detected.It is necessary to strength
作者
章婧
陈海欣
喻梦梦
高颖茵
刘文字
蒋红霞
ZHANG Jing;CHEN Haixin;YU Mengmeng;GAO Yingyin;LIU Wenzi;JIANG Hongxia(National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria/Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2022年第3期483-489,共7页
Chinese Journal of Veterinary Science
基金
国家重点研发计划资助项目(2016YFD0501300)。
关键词
鸡肉源
粪肠球菌
耐药性
毒力基因
chicken
E.faecalis
antimicrobial resistance
virulence genes
