摘要
目的:观察电针“内关”对自发性高血压大鼠(SHR)左心室功能的影响,并初步探究内皮素-1(ET-1)/内皮型一氧化氮合酶(eNOS)的介导作用。方法:选用12周龄雄性京都Wistar(WKY)大鼠6只为正常组;另选12周龄SHR 18只,随机分为模型组、电针组和假电针组,每组6只。电针组选取“内关”进行干预(疏密波,2 Hz/15 Hz,1 mA,持续30 min),每日1次,共8周。假电针组仅于“内关”浅刺,不予电刺激。电针干预结束后,采用超声心动图检测各组大鼠左心室射血分数(LVEF)、左心室短轴缩短率(LVFS),采用右侧颈总动脉插管检测各组大鼠左心室收缩压(LVSP)、左心室舒张期末压(LVEDP)、心率(HR)、左心室内压最大上升速率(+dp/dt_(max))、左心室内压最大下降速率(-dp/dt_(max));采用ELISA法检测各组大鼠血清ET-1含量,Western blot法检测各组大鼠左心室心肌组织内皮素受体A(ETAR)、eNOS蛋白表达情况。结果:与正常组比较,模型组大鼠LVEF、LVFS、+dp/dt_(max)/LVSP、-dp/dt_(max)/LVSP均降低(P<0.01,P<0.05);LVSP、LVEDP、+dp/dt_(max)、-dp/dt_(max)均升高(P<0.01)。与模型组比较,电针组LVEF、LVFS、+dp/dt_(max)/LVSP、-dp/dt_(max)/LVSP均升高(P<0.01,P<0.05);LVSP、LVEDP均降低(P<0.01)。与正常组比较,模型组血清ET-1含量、心肌组织ETAR蛋白表达均升高(P<0.01),eNOS蛋白表达降低(P<0.01);与模型组比较,电针组血清ET-1含量、心肌组织ETAR蛋白表达均降低(P<0.05),eNOS蛋白表达升高(P<0.05)。结论:电针干预可能是通过上调心肌组织eNOS蛋白表达,下调血清ET-1含量、心肌组织ETAR蛋白表达实现对高血压性心功能损害的缓解作用。
Objective To observe the effect of electroacupuncture(EA)at"Neiguan"(PC 6)on cardiac function of ventriculus sinister in rats with spontaneously hypertensive(SHR),and to explore the mediation effect of endothelin-1(ET-1)/endothelial nitric oxide synthase(eNOS).Methods Six 12-week-old male Wistar Kyoto(WKY)rats were taken as the normal group.Eighteen 12-week-old SHR were randomly divided into a model group,an EA group and a sham EA group,6 rats in each group.The rats in the EA group were treated with EA(disperse-dense wave,2 Hz/15 Hz in frequency,1 mA in current intensity)at"Neiguan"(PC 6),30 min each time,once a day for 8 weeks.The rats in the sham EA group were treated with superficial needling at"Neiguan"(PC 6)with no electrical stimulation applied.After treatment,the left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)were tested by echocardiographic analysis.The left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),heart rate(HR),the maximum rate of increase/decrease of left ventricular pressure(±dp/dt_(max))were detected.The serum content of ET-1 was detected by ELISA.Western blot was used to evaluate the expression of ETAR,eNOS in myocardial tissue of left ventricular.Results Compared with the normal group,LVEF,LVFS,+dp/dt_(max)/LVSP and-dp/dt_(max)/LVSP were decreased(P<0.01,P<0.05),while LVSP,LVEDP,+dp/dt_(max) and-dp/dt_(max) were increased(P<0.01)in the model group.Compared with the model group,LVEF,LVFS,+dp/dt_(max)/LVSP and-dp/dt_(max)/LVSP were increased(P<0.01,P<0.05),and LVSP and LVEDP were decreased(P<0.01)in the EA group.Compared with the normal group,the serum content of ET-1 and the expression of ETAR in myocardial tissue were increased(P<0.01),whereas expression of eNOS was decreased(P<0.01)in the model group.Compared with the model group,the serum content of ET-1 and the expression of ETAR in myocardial tissue were decreased(P<0.05),whereas expression of eNOS was increased(P<0.05)in the EA group.Conclusion EA intervention
作者
辛娟娟
高俊虹
刘群
赵玉雪
周晨
喻晓春
XIN Juan-juan;GAO Jun-hong;LIU Qun;ZHAO Yu-xue;ZHOU Chen;YU Xiao-chun(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国针灸》
CAS
CSCD
北大核心
2022年第6期647-653,共7页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金资助项目:81804211、81774439
中国中医科学院基本科研业务费优秀青年科技人才项目(创新类)培养专项项目:ZZ13-YQ-065。
关键词
原发性高血压
电针
左心室功能
内皮素-1
内皮型一氧化氮合酶
essential hypertension
electroacupuncture
left ventricular function
endothelin-1(ET-1)
endothelial nitric oxide synthase(eNOS)
