摘要
为了探究太赫兹波抑制K562细胞存活率基因表达变化的整体情况,揭示其可能的关键信号通路,利用太赫兹波干预K562细胞,基于RNA测序分析基因表达变化的整体差异,通过生物信息学方法分析差异表达基因的富集与聚类特征。以一定的条件筛选出1131个差异表达基因,包括上调表达基因216个,下调表达基因915个。基因本体论富集分析结果表明,差异表达基因主要富集在DNA结合转录激活剂活性、RNA聚合酶与DNA结合、转录因子活性、蛋白质折叠、共价染色质修饰和组蛋白修饰等过程中。京都基因和基因组百科全书的信号通路分析结果表明,差异表达基因主要富集在丝裂原活化蛋白激酶、C型凝集素受体、Notch、Janus激酶信号转导及转录激活因子和转化生长因子-β等与K562细胞增殖和凋亡密切相关的信号通路。本研究对揭示太赫兹波对生物体效应的影响具有一定的参考价值。
To explore the overall situation of terahertz wave inhibition of K562 cell viability gene expression and reveal its possible key signaling pathways.In this study,terahertz wave was used to intervene K562 cells,RNA sequencing technology was used to analyze the overall differences in gene expression changes,and bioinformatics method was used to analyze the enrichment and clustering characteristics of differentially expressed genes.Under certain conditions,1131 differentially expressed genes were screened,among which 216 expressed genes were upregulated and 915 expressed genes were down-regulated.The results of gene ontology enrichment analysis showed that differentially expressed genes were mainly concentrated in DNA-binding transcriptional activator activity,RNA polymerase-DNA binding,transcription factor activity,protein folding,covalent chromatin modification and histone modification,etc.The signaling pathway analysis results of Kyoto Encyclopedia of Genes and Genomes showed that the differentially expressed genes were mainly concentrated in mitogen-activated protein kinase,C-type lectin receptor,Notch,Janus-activated kinase singal transducers and activators of transcriprion and transforming growth factor-β signaling pathways which are closely related to the proliferation and apoptosis of K562 cells.The study has certain reference value for revealing the effect of terahertz wave on organism.
作者
陈中
尚广彬
傅柳
柳歌
陈宣
张琦
卢晓南
Chen Zhong;Shang Guangbin;Fu Liu;Liu Ge;Chen Xuan;Zhang Qi;Lu Xiaonan(Research Center for Differention and Development of TCM Basic Theory,Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China;Jiangxi Province Key Laboratory of TCM Etiopathogenisis,Nanchang,Jiangxi 330004,China;Experimental Center of Basic Medicine,Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2022年第7期269-278,共10页
Laser & Optoelectronics Progress
基金
江西省教育厅科学基金(GJJ180681)。
