摘要
目的:研究JAK2V617F基因突变与骨髓增殖性肿瘤中细胞增殖及凝血功能的关系。方法:选择在本院诊断为BCR-ABL阴性的骨髓增殖性肿瘤患者,检测JAK2V617F基因突变情况并根据检测结果分为突变阳性组和突变阴性组。检测骨髓液中JAK2/STATs信号通路分子、细胞增殖基因的表达量及外周血中凝血功能的指标。结果:突变阳性组骨髓增殖性肿瘤中p-JAK2、p-STAT3、p-STAT5、Survivin、C-myc、CyclinD1、ASXL1的蛋白表达量显著高于突变阴性组,外周血中PT、APTT的水平显著低于突变阴性组,TT、FIB的水平与突变阴性组患者无显著性差异。结论:骨髓增殖性肿瘤中JAK2V617F基因突变能够促进细胞增殖并造成血液高凝状态。
Objective： To study the relationship of JAK2V617F gene mutation with cell proliferation and coagulation function in myeloproliferative neoplasms.Methods： Patients who were diagnosed with BCR-ABL-negative myeloproliferative neoplasms in AnYang Area Hospital between June 2014 and August 2016 were selected, JAK2V617F gene mutation was detected, and according to the test results, the patients were divided into mutation-positive group and mutation-negative group.The expression of JAK2/STATs signaling pathway molecules, cell proliferation genes in Bone marrow fluid as well as the coagulation function indexes in peripheral blood were detected.Results： p-JAK2, p-STAT3, p-STAT5, Survivin, C-myc, CyclinD1, ASXL1 protein expression in myeloproliferative neoplasms of mutation-positive group were significantly higher than those of mutation-negative group, and PT and APTT levels in peripheral blood were significantly lower than those of mutation-negative group while TT and FIB levels were not significantly different from those of mutation-negative group.Conclusions： JAK2V617F gene mutation in myeloproliferative neoplasms can promote cell proliferation and cause hypercoagulable state.
出处
《海南医学院学报》
CAS
2017年第10期1421-1423,共3页
Journal of Hainan Medical University
基金
濮阳市科技攻关(130638)~~
