摘要
目的 研究程序性细胞死亡蛋白2(programmed cell death protein 2,PDCD2)过表达对T淋巴细胞株Jurkat及HIV潜伏感染T细胞株ACH2凋亡的影响。方法 用T淋巴细胞株Jurkat及HIV潜伏感染T细胞株ACH2作为细胞模型,采用慢病毒转染技术分别在上述细胞株过表达PDCD2基因,在未刺激和肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor (TNF)-related apoptosis-inducing ligand, TRAIL)刺激24 h后,经PE-Annexin V、7-AAD检测细胞凋亡的情况。结果 在无刺激剂的情况下,Jurkat和ACH2细胞的凋亡率并未因细胞内PDCD2表达量的增加而改变;在TRAIL刺激下,与对照组相比,高表达PDCD2基因的Jurkat和ACH2细胞凋亡率显著增高,有统计学差异(P<0.05)。结论 PDCD2能促进TRAIL刺激下T细胞及HIV潜伏感染T细胞的凋亡水平。
Objective To investigate apoptosis after PDCD2 overexpression in Jurkat and ACH2 cell lines. Methods Jurkat and ACH2 cell lines were used in this study. PDCD2 was stably expressed in the cell lines by lentivirus infection. Apoptosis was determined by flow cytometry after 24 hours of TRAIL treatment. Results Apoptosis was not significantly increased in Jurkat or ACH2 cells stably expressing PDCD2 compared with untransfected control cells. However, compared with the control group without PDCD2 overexpression, apoptosis was significantly higher in PDCD2-Jurkat and PDCD2-ACH2 cells induced by TRAIL(P<0.05). Conclusions PDCD2 promotes apoptosis of T cell and HIV latent-infected T cells induced by TRAIL.
作者
余雯惠
杨晨波
丛喆
薛婧
YU Wenhui;YANG Chenbo;CONG Zhe;XUE Jing(Comparative Medicine Center,Peking Union Medical College(PUMC)&Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences(CAMS),NHC Key Laboratory of Human Disease Comparative Medicine,Key Laboratory of Human Diseases Animal Models,State Administration of Traditional Chinese Medicine,Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases,Beijing 100021,China)
出处
《中国比较医学杂志》
CAS
北大核心
2022年第5期1-6,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(81971944)
中国医学科学院医学与健康科技创新工程重大协同创新项目(2021-I2M-1-037)。
