摘要
目的探索三七粉活血作用的质量标志物。方法采集家兔血浆,采用体外凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、纤维蛋白原(FIB)检测试剂盒观察三七粉主要成分对凝血系统的影响;收集家兔富含血小板血浆,以二磷酸腺苷(ADP)诱导血小板聚集,观察三七粉主要成分对血小板聚集率的影响;取家兔主动脉条,置于体外组织灌流系统中,以去氧肾上腺素诱导血管条收缩,观察三七粉主要成分对血管平滑肌收缩功能的影响。结果以蒸馏水溶解的三七总皂苷(PNS),与水溶剂组比较,可显著延长TT、APTT、PT,降低FIB含量(P<0.05、P<0.01),降低ADP诱导的血小板聚集率(P<0.01)。以5%二甲基亚砜(DMSO)溶解的单体成分,与5%DMSO溶剂组比较,人参皂苷F_(2)、人参皂苷Rb_(2)、人参皂苷Rb_(3)、人参皂苷Rk1、三七皂苷R1、人参皂苷Rg_(2),可显著延长TT(P<0.05,P<0.01);人参皂苷F_(2)、人参皂苷Rd、人参皂苷Rb_(2)、人参皂苷Rg_(2)、人参皂苷Rb1、槲皮素可延长APTT(P<0.05);人参皂苷Re、槲皮素、人参皂苷F_(2)、人参皂苷Rb_(3),可显著延长PT(P<0.05);槲皮素、人参皂苷F_(2)、人参皂苷Rg_(1)、人参皂苷Rg_(3)、人参皂苷Rb_(2)、人参皂苷Rb1、人参皂苷Rb_(3)、三七皂苷R1、人参皂苷Rg_(2)可显著降低血浆FIB含量(P<0.05);人参皂苷Rg_(1)、人参皂苷Rh1、人参皂苷Rb_(2)、人参皂苷Rg_(2)、三七皂苷R1、人参皂苷F_(2)、人参皂苷Rg_(3)、人参皂苷Rd和人参皂苷Rb1可显著降低ADP诱导的血小板聚集(P<0.05);人参皂苷Rg_(3)、人参皂苷Rh1、人参皂苷Rd、三七皂苷R1、人参皂苷Rk1和人参皂苷Rg_(2)可明显扩张血管动脉条(P<0.05,P<0.01)。结论三七粉活血作用表现在抗凝、抗血小板聚集、扩血管等方面,人参皂苷F_(2)、人参皂苷Rg_(2)、人参皂苷Rg_(3)、三七皂苷R1、人参皂苷Rb1、人参皂苷Rb_(2)、人参皂苷Rb_(3)、人参皂苷Rd、人参皂苷Rg_(1)、槲皮
Objective To explore the quality markers of Panax notoginseng Powder(PNP)in promoting blood circulation.Methods The rabbit plasma was collected and the effects of main components of PNP on coagulation system were observed by using in vitro detection kits of thrombin time(TT),activated partial thrombin time(APTT),prothrombin time(PT)and fibrinogen(FIB).Platelet rich plasma was collected from rabbits and platelet aggregation was induced by adenosine diphosphate(ADP)to observe the effect of main components of PNP on platelet aggregation rate.Rabbit aortic strips were taken and placed in the tissue perfusion system in vitro,and the vasoconstriction of the strips was induced by norepinephrine to observe the effect of main components of PNP on the vasoconstriction function of vascular smooth muscle.Results Compared with the water solvent group,PNS dissolved in distilled water could significantly prolonged TT,APTT,PT,reduced FIB content(P<0.05 and P<0.01),and decreased ADP-induced platelet aggregation rate(P<0.01).Panax notoginseng monomers dissolved in 5%dimethyl sulfoxide(DMSO)were compared with 5%DMSO solvent group.The results showed that ginsenoside F_(2),ginsenoside Rb_(2),ginsenoside Rb_(3),ginsenoside Rk1,notoginsenoside R1 and ginsenoside Rg_(2) could significantly prolong TT(P<0.05,P<0.01);ginsenoside F_(2),ginsenoside Rd,ginsenoside Rb_(2),ginsenoside Rg_(2),ginsenoside Rb1 and quercetin could prolong APTT(P<0.05);ginsenoside Re,quercetin,ginsenoside F_(2) and ginsenoside Rb_(3) could significantly prolong PT(P<0.05);quercetin,ginsenoside F_(2),ginsenoside Rg_(1),ginsenoside Rg_(3),ginsenoside Rb_(2),ginsenoside Rb1,ginsenoside Rb_(3),notoginsenoside R1 and ginsenoside Rg_(2) could significantly reduce the content of plasma FIB(P<0.05);ginsenoside Rh1,ginsenoside Rg_(1),ginsenoside Rb_(2),notoginsenoside R1,ginsenoside Rg_(2),ginsenoside F_(2),ginsenoside Rg_(3),ginsenoside Rd,ginsenoside Rg_(1) and ginsenoside Rb1 could significantly reduce ADP-induced platelet aggregation(P<0.05);ginsenoside Rh1,ginsenoside
作者
李新
徐旭
许浚
郭海彪
李晓霞
林娟
张铁军
刘旻
Li Xin;Xu Xu;Xu Jun;Guo Haibiao;Li Xiaoxia;Lin Juan;Zhang Tiejun;Liu Min(Tianjin Key Laboratory of Quality Markers for Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300301,China;National&Local United Engineering Laboratory of Modern Preparation and Quality Control Technology of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300301,China;Guangzhou Baiyun Mountain and Hutchison Whampoa Ltd.,Guangzhou 510515,China;College of Graduate School,North China University of Science and Technology,Tangshan 063200,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2022年第1期35-46,共12页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会重点项目(81830111):基于“成分构成-靶标网络-生物效应”多维整合的中药质量标志物发现与确认
负责人:许海玉
国家中医药管理局国际合作司中医药国际合作专项(0610-2140NF020630):中国-德国中药与植物药创新研发国际合作基地
负责人:刘昌孝
广州市科学技术协会-广州市院士专家工作站(600180):三七粉质量标志物研究,负责人:王德勤。
关键词
三七粉
活血
质量标志物
三七皂苷
人参皂苷
槲皮素
Panax notoginseng Powder
Promoting blood circulation
Quality markers
Panax notoginseng saponins
Ginsenoside
Quercetin
