摘要
土壤微生物在森林生态系统中起着重要作用,为了解喀斯特常绿落叶阔叶混交林土壤真菌多样性,采用IlluminaHiseq高通量测序技术对木论喀斯特森林土壤真菌群落多样性进行了初步研究。研究结果表明:在82个采样点内,随着采样点增加,检测出不同分类水平的土壤真菌类群逐步增多;土壤样点数达到82个时,检测出的土壤真菌类群达6门32纲126目336科886属和132616个种(OTU);82个土壤样品中所检测出的真菌类群平均有5.41门22.13纲71.09目144.89科216.66属和1256.44个OTU,其中门、纲、目分类水平上的优势类群(所占比例)分别为子囊菌门(Ascomycota)(50.09%)、接合菌纲(Zygomycota)(34.54%)、被孢霉目(Mortierellales)(22.65%)。取样数量对所检测土壤真菌类群数据有显著影响,在门、纲、目、科的分类水平上,1个、15个、48个、73个样品基本上可以代表82个样品检测出来不同分类水平的真菌多样性;在属和OTU的分类水平上,当样品数达到82个时检测出来的响应类群数仍在不断增加。该研究为进一步了解喀斯特森林土壤真菌多样性与植物多样性和生境的关系提供了基础。
Soil microbes play essential roles in forest ecosystems. To understand the diversity of soil fungal in karst forest,we investigated soil fungal community structure in the Mulun karst evergreen and deciduous broad-leaved mixed forest using the technology of Illumina Miseq. The results showed that in the 82 soil sampling sites, the number of fungal taxa detected at different classification levels increased with the increasing number of sampling points. When all 82 samples were considered,the number of relative fungal groups include 6 phyla, 32 classes, 126 orders, 336 families, 886 genera, and 132616operational taxonomic units(OTUs). The mean values of relative fungal taxa in the 82 samples were 5.41 phyla, 22.13classes, 71.09 orders, 144.89 families, 216.66 genera, and 1256.44 OTUs. At the classification level of phylum, class, and order, the dominant groups were Ascomycota(50.09%), Zygomycota(34.54%), Mortierellales(22.65%), respectively. These preliminary results show that the soil has a relatively high level of fungal diversity in the karst evergreen and deciduous broad-leaved mixed forest, which lays the foundation for further studies on the relationship between soil fungal diversity and plant diversity and other related scientific questions.
作者
谷俊锟
曾馥平
宋同清
彭晚霞
苏樑
杜虎
GU Junkun;ZENG Fuping;SONG Tongqing;PENG Wanxia;SU Liang;DU Hu(Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academic of Sciences,Changsha,Hunan 410125,China;Guangxi Key Laboratory of Karst Ecological Processes and Services,Huanjiang Observation and Research Station of Karst Ecosystem,Huanjiang,Guangxi 547100,China;College of Resources and Environment,Hunan Agricultural University,Changsha 410125,China)
出处
《生态科学》
CSCD
2022年第3期54-61,共8页
Ecological Science
基金
广西重点研发计划项目(桂科AB17129009)
国家自然科学基金项目(31770495,31870712,31971487)
河池市特聘专家项目。
关键词
土壤真菌多样性
高通量测序
采样数
森林
喀斯特生态系统
soil fungal diversity
high-throughput sequencing
number of samples
forest
karst ecosystem
