摘要
目的:水凝胶因与细胞外基质具有极其相似的性质在生物医用领域被广泛应用,而传统水凝胶的生物相容性和复杂的制备工艺限制了其应用的发展。本研究以天然多糖(壳聚糖和海藻酸钠)为原料设计一种自修复水凝胶的制备方案,拓展其在生物医用领域的应用。方法:首先,采用高碘酸钠氧化海藻酸钠(OSA)的方法制备带双醛基的OSA;然后,壳聚糖(CS)分子链上的氨基与OSA分子链上的醛基发生席夫碱反应形成亚胺键得到CS-OSA水凝胶。结果:红外光谱、扫描电镜和旋转流变仪表征结果表明,OSA和CS能够快速点击交联形成水凝胶,凝胶时间小于10 s,具有三维多孔结构、优异的机械性能和自修复性能。抑菌实验证明了该水凝胶对大肠杆菌和金黄色葡萄球菌具有明显的抑菌性能,体外细胞培养实验证明该水凝胶没有明显的细胞毒性,动物皮肤创面模型实验证明该水凝胶具有促创面愈合的效果。结论:CS-OSA水凝胶具有良好的生物相容性和促创面愈合效果,在生物医用领域具有很好的应用前景。
Objective:Hydrogels have been widely used in the field of biomedicine because of their similar properties to extracellular matrix.However,the poor biocompatibility and complex preparation process of traditional hydrogels has limited their application.In this study,we proposed a strategy to design and prepare an self-healing hydrogel based on natural polysaccharides(chitosan and sodium alginate)by in-situ self-crosslinking,intending to expanding its application in biomedical field.Methods:Firstly,oxidized sodium alginate(OSA)with dialdehyde groups was obtained by oxidizing sodium alginate with sodium periodate.Secondly,the amino groups on the chitosan(CS)molecular chain and the aldehyde groups on the OSA molecular chain was reacted to form imine bonds through Schiff base reaction,thus to yield CS-OSA hydrogel under mild conditions.Results:The infrared spectroscopy,scanning electron microscope(SEM)and rotational rheometer were used to characterized and studied the properties of CS-OSA hydrogel.The results proved that CS and OSA formed hydrogel by quick click cross-linking.The gelation time was less than 10 s,and the obtained hydrogel showed a three-dimensional porous network structure,excellent mechanical properties and self-healing properties.The hydrogel had distinct antibacterial properties in Escherichia coli and Staphylococcus aureusare in antibacterial test.Furthermore,the hydrogel showed no obvious cytotoxicity in cell culture.The CS-OSA hydrogels exhibited wound-healing performance in animal skin wound model.Conclusion:CS-OSA hydrogel has good biocompatibility and wound-healing performance.Therefore,the hydrogel has a good application prospect in biomedical field.
作者
耿志杰
于珊
曾志文
黄骏
国翠平
鲁道欢
裴大婷
GENG Zhijie;YU Shan;ZENG Zhiwen;HUANG Jun;GUO Cuiping;LU Daohuan;PEI Dating(Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, Guangdong, China;National Engineering Research Center for Healthcare Devices, Guangzhou 510500, Guangdong, China)
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2022年第3期322-331,共10页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省基础与应用基础研究基金项目(2019A1515110207)
广州市科技计划项目(202002030381)
广东省科学院建设国内一流研究机构行动专项项目(2019GDASYL-0501003,2021GDASYL-20210103030)。
关键词
水凝胶
亚胺键
自修复
点击交联
抑菌性能
hydrogel
imine bond
self-healing
click cross-linking
antibacterial properties
