摘要
通过对几种天然高分子聚合材料及其复合物的形貌、透光性、机械强度和化学稳定性等性能的比较,综合选择最优的材料作为光合细菌固定化载体,并对固定化条件进行优化.结果表明,2%琼脂和2%卡拉胶在以1∶1混合时制备的固定化颗粒的性能最优,最适宜的固定化条件为在2%的KCl中固定60 min,细菌包埋量为40 mL(菌液)/6 mL(固定化材料).随后,以模拟预水解液(PHL)作为底物,采用琼脂-卡拉胶固定光合细菌形成微球,对其产氢性能进行分析.结果表明,第二次循环的产氢量为第一次的1.8倍,木糖利用率最高可达99.4%,苯酚降解率最大为28.5%,并且系统的pH相对平稳.固定化光合细菌在连续六个循环中仍能保持较高的产氢性能,表明固定化微球具有稳定的重复利用性,具有良好的应用前景.
Based on the comparison of light transmittance,mechanical strength and chemical stability of several natural polymer materials and their composites,the optimal material was selected as the carrier of photosynthetic bacteria immobilization,and the immobilization conditions were optimized.The results showed that the performance of immobilized particles prepared by mixing 2%agar and 2%carrageenan at a ratio of 1∶1 was the best.The most suitable immobilization conditions were fixed in 2%KCl for 60 minutes,and 40 mL of bacteria were embedded in 6 mL of carrier.Subsequently,agar-carrageenan was used to immobilize photosynthetic bacteria to form microspheres and the hydrogen production performance was analyzed in a simulated pre-hydrolyzed solution(PHL).The results showed that the hydrogen production of the second cycle was 1.8 times that of the first cycle,the utilization rate of xylose and the degradation rate of phenol were up to 99.4%and 28.5%,respectively,and the pH of the system was relatively stable.The immobilized photosynthetic bacteria can still maintain high hydrogen production performance in six cycles,indicating that the immobilized microspheres have stable reusability and have good application prospects.
作者
陈朵
王雪青
张安龙
罗清
王浩楠
CHEN Duo;WANG Xue-qing;ZHANG An-long;LUO Qing;WANG Hao-nan(College of Bioresources Chemical and Materials Engineering, National Demonstration Center for Experimental Light Chemistry Engineering Education, Shaanxi University of Science & Technology, Xi′an 710021, China;School of Environmental Science and Engineering, Shaanxi University of Science & Technology, Xi′an 710021, China)
出处
《陕西科技大学学报》
北大核心
2021年第2期16-22,49,共8页
Journal of Shaanxi University of Science & Technology
基金
陕西省科技厅自然科学基金项目(2017JQ5097)
陕西科技大学博士科研启动基金项目(2016QNBJ-04)。
关键词
琼脂-卡拉胶
光合细菌
固定化
预水解液
制氢
agar-carrageenan
photosynthetic bacteria
immobilizition
pre-hydrolysate
hydrogen production
